引进小麦种质材料的高分子量谷蛋白亚基分析  

Analysis on the HMW-GS Components of Introduced Wheat Varieties

作　　者：刘争辉[1] 刘祎[1] 何旭[1] 杨起简[2] Dragovich A.Yu. Volodymyr S.Kravets 

机构地区：[1]北京农学院植物科技学院,北京102206 [2]北京农学院生物技术系,北京102206 [3]俄罗斯科学院瓦维洛夫遗传所,莫斯科119991 [4]乌克兰科学院石油有机化学研究所,基辅02094

出　　处：《中国农学通报》2009年第22期36-41,共6页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划"牧草倍性育种技术研究"(2008-BADB3B03);北京市科委国际合作项目"几种重要农业资源的引进和利用"(2007N16);科技部政府间合作项目"中国小麦醇溶蛋白多态性研究及利用"(№CR-11-19);科技部国际科技合作项目"逆境胁迫下小麦抗性机理的研究"(№cu-08-05)

摘　　要：为了有效利用多年来引自俄罗斯及中亚地区的小麦资源,了解引进材料的遗传基础,特别是品质基础,采用SDS-PAGE技术对102份小麦材料的高分子量谷蛋白亚基(HMW-GS)组成进行了分析。结果表明,参试材料中共检测到11种HMW-GS类型,Glu-A1位点上有1、2*、Null,Null位点相对比较多,为40.20%;Glu-B1位点上有7、7+8、7+9、6+8、17+18,以7+9为主要类型(59.80%);Glu-D1位点有2+12、2+12’、5+10三种类型,其中5+10所占比例为51.96%。参试材料共检测到16种亚基组合,其中"Null,7+9,2+12"所占比例较大,为25.5%。值得一提的是,参试材料中品质评分为10分的材料有22个,9分的有29个。这些材料有可能会成为比较有价值的品质改良中间材料。In order to make good use of foreign varieties, and to realize the genetics base, especially the quality traits of these introduced materials. HMW-GS compositions of 102 wheat cuhivars from Russia and middle Asia area were analyzed by SDS-PAGE. The results showed that the number of allelic variations was eleven. At Glu-A1 locus, three alleles were detected, which encoded subunit 1, 2＊and Null respectively and the major subunit was Null with the frequency of 40.20%; At Glu-B1 locus, five alleles were detected, which encoded7, 7＋8, 7＋9, 6＋8, 17＋18, the 7＋9 was the major subunit pattern（59.80%）. At Glu-D1 locus, there were three alleles which encoded 2＋ 12, 2＋ 12＇ and 5＋ 10 respectively were detected, and there were 51.96% materials took the subunit 5＋ 10. Sixteen subunit combination patterns were identified and the major pattern was ＂Null, 7＋9, 2＋12＂with the frequency of 25.5%. It is worth mentioning that there were twenty two cuhivars with the score of 10 and twenty nine varieties with the score of 9 according to Payne＇s scoring system. It was showed that these materials may be a promising resource for improving the bread-making quality of wheat in China.

关 键 词：小麦 品质 高分子量谷蛋白 十二烷基磺酸钠-聚丙稀酰胺凝胶电泳 

分 类 号：S512.1[农业科学—作物学] S331