β-榄香烯乳联合照射对DNA-PKcs基因表达及凋亡的影响  

The Influence of β-elemene Combined with Radiotherapy on the Expression of DNA-PKcs mRNA and Apoptosis

作　　者：张卓[1] 邹丽娟[1] 田莹莹[2] 

机构地区：[1]大连医科大学附属二院肿瘤放疗科,116023 [2]青岛市肿瘤医院肿瘤科,266042

出　　处：《实用癌症杂志》2009年第6期555-559,共5页The Practical Journal of Cancer

基　　金：国家自然科学基金(编号:30770646)

摘　　要：目的探讨β-榄香烯乳影响肺腺癌A549细胞凋亡及DNA-PKcs基因表达的放射增敏机制。方法实验分为对照组、单纯照射组(4Gy照射)、单纯药物组(10μg/ml、20μg/mlβ-榄香烯乳)、药物+照射组(10μg/ml、20μg/mlβ-榄香烯乳+4Gy照射)。采用四氮唑蓝比色分析法(MTT法)检测β-榄香烯乳对肺腺癌A549细胞的半数抑制浓度(IC50);克隆形成实验计算细胞存活率;采用流式细胞仪检测细胞周期与凋亡率;逆转录实时荧光定量PCR技术(RT-PCR)检测细胞DNA-PKcs基因mRNA表达量。结果MTT法测得β-榄香烯乳对A549细胞的IC50值为120μg/ml;克隆形成实验证明β-榄香烯乳对A549细胞有放射增敏作用;药物+照射组细胞G2/M期比率及凋亡率明显高于单纯照射组及单纯用药组(P<0.05);药物+照射组DNA-PKcs基因mRNA表达量与单纯照射及单纯用药组相比明显下降(P<0.05)。结论β-榄香烯乳可通过促进A549细胞凋亡及抑制DNA-PKcs基因mRNA表达实现对A549细胞放射增敏作用。Objective To explore the mechanism of radiosensitization of β-elemene to lung cell line of A549 from influcing the expression of DNA-PKcs mRNA and apoptosis. Methods Objective To explore the mechanism of radiosensitization of β- elemene to lung cell line of A549 from influcing the expression of DNA-PKcs mRNA and apoptosis. Methods The cells were devided into control group, radiotherapy group （4 Gy radiation）, drug group（ 10 μg/ml,20 μg/mlβ-elemene ）and drug with radiotherapy group （ 10 μg/ml,20 μg/ml β-elemene combined with 4 Gy radiation）. IC50 was detected by MTT method and cell survival rate was detected by colony formation test. Cell cycle and apoptosis were analyzed with flow cytometry. The expression level of DNA-PKcs mRNA was detected by Reverse Transcription-Polymerase Chain Reaction （RT-PCR）. Results MTTassay showed that IC50 values of A549 cell was 120 p,g/ml. Colony formation test showed that the radiosensitivity of A549 cells could be enhanced by β-elemene. The flow eytometry confirmed that the ratio of G2/M and apoptosis were significantly increased than control group under the effect of β-elemene. The expression level of DNA-PKcs mRNA of 10 μg/ml,20 μg/ml β-elemene with radiother- apy group was declined significantly than those of the control group （ P 〈 0.05 ）. Conclusion The radiosensitization effect of β- elemene to A549 cell is associated with inducing cell apoptosis and down-regulating the expression level of DNA-PKcs mRNA .

关 键 词：β-榄香烯乳 肺腺癌A549细胞 DNA—PKcs 凋亡 放射增敏 

分 类 号：R734.2[医药卫生—肿瘤]