P14^(ARF)、P16^(INK4a)基因表达与高危型HPV感染的关系  

The Relation of P14^(ARF)、P16^(INK4a) Expression and High-risk Papillomavirus Infection

作　　者：于晓红[1] 熊勇[1] 魏宝秀[1] 李隆玉[1] 舒宽勇[1] 秦贇娜[1] 付秋风[1] 揭由坤[1] 邓颖辉[1] 

机构地区：[1]江西省妇幼保健院,330006

出　　处：《实用癌症杂志》2009年第6期580-583,共4页The Practical Journal of Cancer

基　　金：江西省卫生厅科研基金资助项目(20051151)

摘　　要：目的探讨P14ARF、P16INK4a的基因表达与高危型人乳头瘤病毒DNA(HPV DNA)感染的关系。方法采用免疫组化EnVision法,对130例宫颈鳞癌、宫颈上皮内瘤变60例、20例正常宫颈组织进行P16INK4a、P14ARF蛋白基因检测,并采用原位杂交法检测130例宫颈鳞癌、宫颈上皮内瘤变60例、20例正常宫颈组织中的HPV DNA。结果宫颈鳞癌组中P16INK4a、P14ARF蛋白表达率分别为100.0%(130/130)和96.9%(126/130);在60例宫颈上皮内瘤变组中其表达率分别为85.0%(51/60)、81.7%(49/60);在20例正常宫颈组中其表达率分别为10.0%(2/20)、15.0%(3/20),宫颈鳞癌组显著高于正常对照组(P<0.01);正常宫颈组与宫颈上皮内瘤变及宫颈鳞癌组P16INK4a和P14ARF的表达呈显著差异(P<0.05)。HPV16/18在130例宫颈鳞癌中86例(66.2%)表达阳性;在宫颈上皮内瘤变中39例(65.0%)表达阳性;P16INK4a、P14ARF阳性率HPV DNA阳性宫颈鳞癌、宫颈上皮内瘤变组与HPV DNA阴性宫颈鳞癌、宫颈上皮内瘤变组比较无显著差异(P>0.05)。结论P16INK4a、P14ARF蛋白在宫颈上皮内瘤变及宫颈鳞癌组织中呈高表达,有较高的特异性和敏感性,可作为宫颈癌前病变及宫颈癌的诊断指标。Objective To investigate the relationship between P14ARF、P16INK4a expression and the high-risk human papillomavirus infection. Methods The Envision immunohistochemical method was used to detect P14ARF、P16INK4a in 130 cervical squamous carcinoma,60 cervical intraepithelial neoplasia and 20 normal cervical tissues. The in situ hybridization was used to detect the HPV DNA in the same cases. Results The expression rates of P14ARF、P16INK4a in 130 cervical squamous cell carcinoma, 60 cervical intraepithelial neoplasia,20 normal cervical tissues were 100.0% （130/130） and 96.9% （126/130） ,85.0% （51/ 60） and 81.7% （49/60）, 10.0% （2/20） and 15.0% （3/20） respectively. The expression rates of these markers in cervical squamous cell carcinoma were significantly higher than in normal cervical tissues （ P 〈 0.01 ）. And there were significant difference between the normal cervical tissues and the cervical intraepithelial neoplasia or the cervical squamous carcinoma in the expression rates of P16INK4a and P14ARF（P 〈 0. 05 ）. Among the 130 cervical squamous cell carcinoma 86 were positive of HPV16 and HPVI 8 （66.2%） ;among the 60 cervical intraepithelial neoplasia 39 were positive （65.0%）. The positive rates of P14ARF and P16INK4a had no significant difference between the HPV DNA positive cervical squamous cell carcinoma,cervical intraepithelial neoplasia and the negative ones （P 〉 0.05 ）. Conclusion P14ARF and P16INk4a express highly in cervical intraepithelial neoplasia and cervical squamous cell carcinoma. The two markers have higher specificity and sensitivity and can be used as the valuable parameters to diagnose cervical precancerous lesion and cervical carcinoma.

关 键 词：宫颈肿瘤 P14ARF 蛋白 P16INK4A蛋白 免疫组化 

分 类 号：R737.33[医药卫生—肿瘤]