纸张纤维老化的生物检测方法  

Bioassay for aging paper fiber

作　　者：孟朝阳[1,2] 赵长新[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034 [2]辽宁警官高等专科学校公安技术系,辽宁大连116036

出　　处：《大连工业大学学报》2009年第6期429-432,共4页Journal of Dalian Polytechnic University

基　　金：公安部科技创新项目(2008YYCXLNST201)

摘　　要：探讨了检测纸张纤维老化的纤维素酶酶解生物分析方法。纤维素酶酶解法采用条件实验,分别考察了反应温度、pH值、酶解时间及加酶量对纤维素酶活力的影响趋势,确定了纤维素酶酶解纸张的最佳条件。实验结果表明,在最适pH值为4.8、最适反应温度50℃、纤维素酶解时间60 min、加酶量为10 kU/mL的条件下,不同老化程度的纸张经纤维素酶分解后,利用3,5-二硝基水杨酸法测得的还原糖量呈现上升的趋势,可以此来初步判定纸张纤维的老化程度,确定纸张的相对形成时间,从而建立了用生物方法检测纸张纤维老化程度的方法。该方法与其他检验方法相比,能够区分出纸张相对形成时间较小的样品,达到鉴别的目的。Bioassay for aging paper fiber by evaluating cellulose degradation via cellulase has been studied in this paper. The optimum condition of cellulase hydrolysis was investigated, including temperature, pH, time and the amount of enzyme. The maximal degradation catalyzed by cetlulase was obtained in the case of pH 4.8, temperature 50 ℃, enzymatic time 60 min, and the enzyme concentration 10 kU/mL. The release of sugar after enzymatic hydrolysis showed its relation to the aging degree of paper, which presumably was used to judge the producing time of paper. The small paper is needed for paper identification with the cellulolytic method compared with other test method.

关 键 词：纸张老化 纤维素酶 生物检测 

分 类 号：TS711[轻工技术与工程—制浆造纸工程] Q814.9[生物学—生物工程]