离心法测定细胞与材料之间黏附强度的研究  

Centrifugation Method for Detecting the Adhesion of Cell to Material

作　　者：方凯正[1] 赵东旭[1] 

机构地区：[1]北京理工大学生命学院,北京100081

出　　处：《北京生物医学工程》2009年第4期401-403,411,共4页Beijing Biomedical Engineering

基　　金：973计划课题（2005CB623906）资助

摘　　要：建立一种简单的离心法用以评价细胞与材料之间的黏附强度。改造离心涂片机以适合研究需要；建立离心法测定三种贴壁细胞（HeLa、SH～SY5Y、ECV304）在两种材料（聚苯乙烯板、丝素蛋白膜）上的黏附强度，以研究在不同的培养时间下三种贴壁细胞在两种材料上的黏附情况。结果表明在相同的培养时间下，三种贴壁细胞与丝素蛋白膜的黏附强度强于与聚苯乙烯的黏附强度，其黏附强度的大小可以相对量化。结论该离心法检测细胞对生物材料黏附强度的方法简单易行，重复性高；丝素膜材料是一较为理想的生物材料。A simple centrifugation method for detecting the cell＇s adhesion to material was set up in the study. Firstly the centifuge for smearing cell was adapted to suit for detecting the cell＇ s adhesion to material. Then a centrifugal method was studied for detecting the adhesion of 3 kinds of adherent cell（ HeLa, SH-SYSy, ECV304） to two kinds of material（polystyrene plate and silk fibroin film）. The results showed that the adhesion to silk fibroin film of each kind of cell was stronger than that to polystyrene plate in same culture time. And the adhension could be relative quantified, The centrifugal method for detecting the cell＇ s adhesion to material was operated easily and its reproducibility was validated. This experiment indicated that the silk fibroin film was one kind of ideal biomaterial.

关 键 词：离心法 贴壁细胞 聚苯乙烯 丝素蛋白膜 

分 类 号：R318.01[医药卫生—生物医学工程]