子宫颈癌候选抑癌基因Syk表达及其启动子甲基化的测定  

Expression of Syk gene and the methylation of its promoter in cervical carcinoma

作　　者：赵淑萍[1] 孙桂霞[2] 马德花[1] 陈荣辉[1] 

机构地区：[1]青岛大学医学院附属医院妇科,266003 [2]河南大学淮河医院妇产科

出　　处：《中华全科医师杂志》2009年第12期901-903,共3页Chinese Journal of General Practitioners

摘　　要：采用逆转录PCR（RT-PCR）法检测60例宫颈癌、50例宫颈上皮内瘤变（CIN）和20例正常宫颈组织中SykmRNA的表达，采用甲基化特异性PCR（MSP）检测Syk基因启动子甲基化情况。正常宫颈组织和CINⅠ组织中均有Syk基因表达，CINⅡ-Ⅲ组织56％（18／32）、宫颈癌组织35％（21／60）表达；有淋巴结转移的宫颈癌组织表达比例为1／13，无淋巴结转移者为43％（20／47）。宫颈癌组织中Syk基因启动子甲基化率57％（34／60），明显高于CIN组织[18％（9／50），X^2值为7．13，P〈0．01]，正常宫颈组织和CINⅠ组织中均无Syk基因启动子的甲基化，淋巴结转移的宫颈癌组织比例为11／13；Syk基因及其启动子甲基化程度与患者年龄、肿瘤大小、临床分期、组织学类型及病理类型无明显相关性（P〉0．05），与Syk基因表达缺失明显相关（P〈0．05）。Syk基因启动子甲基化可能是导致Syk基因失活的原因之一，可能与宫颈癌发生发展有关。Reverse transcription-PCR and methylation-specific PCR （MSP） were used to determine the expression levels of Syk gene and the methylation status of its promoter in tissue samples from 60 patients with cervical cancer, 50 patients with cervical intraepithelial neoplasia （CIN） , and 20 normal controls. We also analyzed the association of the methylation status and expression levels of Syk gene with linicopathological features of patients. The expression rates of Syk gene in 20 normal cervical tissue samples and 18 CIN Ⅰsamples were both 100% ; those of CIN Ⅱ - Ⅲand cervical carcinoma were 56% （18/32） and 35% （21/60） respectively. Among cervical carcinoma patients, the expression of Syk mRNA was detected in one out of 13 cases with lymph node metastasis （1/13） and in 20 out of 47 cases with no lymph node metastasis （43%）. The methylation of Syk gene in promoter region was detected in 34 out of 60 cases of cervical carcinoma （57%） ; while there was no methylation in CIN cases. In 13 cases with lymph node metastasis, 11 were found to have the methylation of Syk gene. The methylation rate of Syk promoter in cervical carcinoma was higher than that of CIN tissue（X^2 =7. 13, P 〈0. 01）. The methylation status of Syk gene was correlated with the lymph node metastasis （ P 〈 0. 05 ） , but not with other clinicopathologieal parameters （ P 〉 0. 05 ）. There was a significant correlation between methylation status and expression level of Syk gene （P 〈 0. 05 ）. The hypermethylation leads to silencing of the Syk gene in human cervical carcinoma. Syk hypermethylation may be associated with oncogenesis, metastasis of cervical carcinoma.

关 键 词：宫颈肿瘤 基因 抑制 

分 类 号：R737.33[医药卫生—肿瘤]