微囊化肝细胞移植对急性肝衰竭大鼠肝组织Smac及细胞色素cmRNA表达的影响  

Smac/DIABLO and cytochrome c mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatocytes

作　　者：汪洪姣[1] 徐志伟[1] 陈均亚[1] 林海龙[1] 陈益平[1] 陈永平[2] 

机构地区：[1]温州医学院附属育英儿童医院感染科,325027 [2]温州医学院附属第一医院感染内科

出　　处：《中华临床感染病杂志》2009年第6期341-344,共4页Chinese Journal of Clinical Infectious Diseases

基　　金：浙江省卫生厅项目（20078141）

摘　　要：目的动态观察微囊化肝细胞腹腔移植对急性肝衰竭大鼠肝组织Smac／DIABLO及细胞色素c（cyt—c）mRNA表达的影响。方法D-氨基半乳糖诱导大鼠急性肝衰竭模型，并设微囊化肝细胞移植组、裸肝细胞移植组和对照组。RT—PCR法测定大鼠肝脏凋亡指标Smac／DIABLO和cyt—cmRNA表达情况。样本比较采用重复测量的多元方差分析或单因素方差分析，组间比较采用t检验。结果急性肝衰竭大鼠肝组织中Smac／DIABLO及cyhmRNA的表达较对照组明显增加（F值为4．345、14．821、47．565、42．178、62．961，P值均〈0．05）。裸肝细胞移植组和对照组肝组织中的Smac／DIABLO和cyt—cmRNA表达在48h最高，而微囊化肝细胞移植组的峰值略微前移，在24h最高。结论Smac／DIABLO及cyt—CmRNA的表达随着急性肝衰竭的进程而波动，可以较好地判断肝细胞凋亡情况。Objective To investigate Smac/DIABLO and cytochrome c （cyt-c） mRNA levels in liver tissue of rats with acute hepatic failure treated by microencapsulated hepatoeyte. Methods Acute hepatic failure were induced by intraperitoneal injection of D-galactosamine in rats, and the rats were treated with microencapsulated hepatocytes, free hepatoeytes and physical saline （control）, respectively. Smac/ DIABLO and cyt-c mRNA in liver tissue was detected by RT-PCR and the mRNA expression levels among three groups were compared. Results Smac/DIABLO and eyt-c mRNA levels in liver tissues of rats with acute hepatic failure were higher than those of normal rats （ F = 4. 345, 14. 821, 47. 565, 42. 178 and 62. 961, P 〈0. 05）. The peak values of Smac/DIABLO and cyt-c mRNA expressions in free hepatoeytes and control groups were at 48 h, while that in mieroeneapsulated hepatocytes group was at 24 h. Conclusion Smac/DIABLO and eyt-c mRNA expression is an indicator of apoptosis of hepatocytes.

关 键 词：细胞色素C类 第二个线粒体衍生的半胱氨酸蛋白酶激活剂 肝细胞移植 微囊化肝细胞 

分 类 号：R575[医药卫生—消化系统]