Isolation and Purification of Plantamajoside and Acteoside from Plant Extract of Plantago asiatica L. by High Performance Centrifugal Partition Chromatography  

作　　者：LI Li LIU Chun-ming CHEN Zhao-jie WANG Jing SHI Dong-fang LIU Zhi-qiang 

机构地区：[1]The Central Laboratory, Changchun Normal University, Changchun 130032, P. R. China [2]Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130021, P. R. China [3]Department of Endocrinology of China-Japan Union Hospital, Jilin University, Changchun 130033, P. R. China

出　　处：《Chemical Research in Chinese Universities》2009年第6期817-821,共5页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.30873364);the Natural Science Foundation of Jilin Province of China(Nos.20071102 and 2008-167)

摘　　要：Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.

关 键 词：Plantago asiatica L Plantamajoside ACTEOSIDE HPCPC ESI-MS HPLC-DAD 

分 类 号：O657.7[理学—分析化学] S511[理学—化学]