Preparation of Adenovirus 5 E1A Polyclonal Antibody  

作　　者：SHEN Qiang MENG Fan-kai YU Yin ZHANG Yu RUO Qi 

机构地区：[1]Department of Neurosurgery, First Hospital of Jilin University,Changchun 130021, P. R. China [2]Department of Neurosurgery, the People＇s Hospital of Jilin Province, Changchun 130021, P R. China

出　　处：《Chemical Research in Chinese Universities》2009年第6期891-894,共4页高等学校化学研究（英文版）

摘　　要：The adenovirus E1A proteins are primarily concerned with modulating cellular metabolism to make the cell more susceptible to virus replication. In this study, adenovirus E1A gene was amplified via PCR on the HEK-293 cells genome and cloned into prokaryotic expression vector pRsetB. The recombinant plasmid pRsetB-E1A was expressed in E.coli BL21 and the relative molecular mass（Mr） of expressed fusion protein was approximately 36000. The recombinant protein was purified on a Ni-NIA agarose column and detected by SDS-PAGE and Westem blot. The purified recombinant protein was then injected into rabbits and anti-E1A polyclonal antibody with high titer was obtained.The adenovirus E1A proteins are primarily concerned with modulating cellular metabolism to make the cell more susceptible to virus replication. In this study, adenovirus E1A gene was amplified via PCR on the HEK-293 cells genome and cloned into prokaryotic expression vector pRsetB. The recombinant plasmid pRsetB-E1A was expressed in E.coli BL21 and the relative molecular mass（Mr） of expressed fusion protein was approximately 36000. The recombinant protein was purified on a Ni-NIA agarose column and detected by SDS-PAGE and Westem blot. The purified recombinant protein was then injected into rabbits and anti-E1A polyclonal antibody with high titer was obtained.

关 键 词：ADENOVIRUS E1A Prokaryotic expression Polyclonal antibody 

分 类 号：Q786[生物学—分子生物学] Q782