小干扰RNA抑制PTTG基因对人胰腺癌细胞PANC-1侵袭能力的影响  

Silence of PTTG gene by RNA interference inhibiting the invasion of human pancreatic cancer cell line PANC-1

作　　者：王宁[1] 王冠军[1] 刘洁[2] 

机构地区：[1]吉林大学第一医院肿瘤中心,吉林长春130021 [2]中国医科大学实验技术中心,沈阳110001

出　　处：《中国实验诊断学》2009年第11期1527-1530,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的研究PTTG基因沉默对胰腺癌细胞侵袭力的影响,探讨其与胰腺癌细胞侵袭性的关系及调控机制。方法阳离子脂质体转染PTTG siRNA对PANC-1中的PTTG基因进行干扰;利用RT-PCR和Western blot技术检测其基因沉默效应;通过Transwell小室法测定PANC-1细胞侵袭能力的改变。结果转染PTTG siRNA后,PANC-1细胞中PTTG的mRNA转录和蛋白表达受抑,显著低于未经处理的PANC-1细胞(P<0.01)。利用PTTG siRNA对PTTG进行RNA干扰后,PANC-1细胞的侵袭能力下降。结论设计的PTTG siRNA能有效抑制体外培养PANC-1细胞中PTTG的mRNA转录和蛋白表达水平,实现其基因沉默效应。PTTG与人胰腺癌的侵袭能力密切相关,其表达水平下降导致细胞侵袭能力降低。Objective To investigate the effect of small interference RNA （siRNA） targeting PTTG gene on the invasion and adhesion ability of pancreatic cancer cell line Panc-1. Methods The chemically synthesized PTTGsiRNA was transferred into cultured Panc-1 cell in vitro by cationic liposome Lipofectamine. RT-PCR and western blot technique were used to test the efficiency of inhibition. The effect on invasion ability after RNA interference was measured by transwell chamber experiment. Results The designed PTTGsiRNA could knock down the transcription and expression of PTTG gene. The difference between the siRNA group and control groups was significant （ P 〈 0. 01 ） ; the invasion ability weakened obviously after PTTG gene silence. Conclusion The siRNA fragment targeting PTTG gene could efficiently make PTTG gene silencing. PTTG gene has a close relationship with the invasion ability of pancreatic, which decreased with the inhibition of PTTG expression.

关 键 词：胰腺癌 垂体瘤转化基因 RNA干扰 

分 类 号：R735.9[医药卫生—肿瘤]