肝癌患者外周血CD4＋CD25＋调节性T细胞的变化及其意义  

The increase of CD4＋CD256 T cells in peripheral blood may be related to immune suppression and tumor progression in liver cancer patients

作　　者：冯茂辉[1] 谢伟[1] 王维娜[2] 余佳莉[1] 段丽[1] 李春光[1] 程伏林[1] 汪付兵[1] 

机构地区：[1]武汉大学中南医院肿瘤外科肿瘤生物学行为湖北省重点实验室,430071 [2]黄石理工学院护理学系

出　　处：《中华实验外科杂志》2009年第12期1637-1638,共2页Chinese Journal of Experimental Surgery

基　　金：湖北省科技攻关计划资助项目（2005AA304805、2006A-A301B59）;湖北省自然科学基金资助项目（2004ABA159）;武汉市科技攻关计划重点项目（200860423230）

摘　　要：目的探讨应用流式细胞术检测肝癌患者外周血中CIM＋CD25＋调节性T细胞的变化及意义。方法应用三色免疫荧光流式细胞仪测定37例肝癌患者及30例肝硬化患者外周血T细胞亚群CD4＋CD25＋／CD4＋比值。采用酶联免疫吸附试验（ELISA）法检测外周血中转化生长因子-β1（TGF-β1）的表达水平。结果肝癌患者外周血CD4＋CD25＋／CD4＋比值较肝硬化患者显著增高，两者比较差异有统计学意义（P〈0．05）；肝癌患者外周血中CD4＋CD25＋T细胞水平与肝癌原发肿瘤的大小、TGF-β1呈正相关（P〈0．05）。结论肝癌患者外周血中CD4＋CD25＋调节性T细胞增多，对肝癌患者具有免疫抑制作用。Objective To evaluate the changes of CD4 ＋ CD25 ＋ T cells in peripheral blood of liver cancer patients, and observe the relationship between CD4 ＋ CD25 ＋ regulatory T cell and the clinicopathologie characters. Methods 37 patients with liver cancer,30 patients with hepatic cirrhosis were included in this study. The proportion of CD4 ＋ CD25＋ T cells population in total T cells was evaluated by flow cytometry analysis. The cytokine production （ TGF-β1 ） was measured by ELISA. Results The population of CD4 ＋ CD25 ＋ T cells in peripheral blood from hepatic carcinoma patients was significantly higher in comparison with that in hepatic cirrhosis patients （ P 〈 0.05 ）. The CD4 ＋ CD25 ＋ T cells population in liver cancer patients was positively correlated with the cancer size and TGF-β1 level （ P 〈 0.05 ）. Conclusion CD4 ＋ CD25 ＋ regulatory T cell is prevalent in liver cancer. It may be responsible for immune suppression in liver cancer patients.

关 键 词：CD4+CD25+调节性T细胞 肝癌 流式细胞术 免疫抑制 

分 类 号：R735.7[医药卫生—肿瘤]