β1整合素表达下调对结肠癌肝转移的抑制作用  

Inhibiting effect of down-regulation of integrin-β1 expression on hepatic metastasis of human colon carcinoma

作　　者：张建立[1,2] 高军[2] 谭晓杰[2] 王敏[1] 王欣[1] 秦仁义[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科,武汉430030 [2]青岛大学医学院附属医院普外科

出　　处：《中华实验外科杂志》2009年第12期1655-1657,共3页Chinese Journal of Experimental Surgery

基　　金：山东省自然科学基金资助项目（Y2007C030）

摘　　要：目的探讨β1整合素表达下调对人结肠癌细胞（HT-29）肝转移的抑制作用。方法采用脂质体将β1整合素反义寡核苷酸（ASODN）转染到HT-29细胞内，逆转录-聚合酶链反应（RT—PCR）和Westernblot检测β1整合素的表达。Transwell侵袭室方法检测HT-29体外侵袭转移能力。裸鼠开腹于脾脏被膜下注射转染的HT-29建立肝转移模型，30d后处死裸鼠，计算肝转移癌的数量与大小，免疫组织化学（SP法）观察转移癌中β1整合素的表达。结果与对照组比较，实验组β1整合素mRNA和蛋白表达水平明显降低（P〈0．05）；Transwell体外侵袭实验实验组迁移的细胞数明显减少（P〈0．05）；体内裸鼠肝转移实验组转移癌数量（4．00±0．93）个／只、平均体积（10．10±6．50）mm^3／只、平均重量（0．0440±0．0008）g／只、免疫组织化学肝转移癌中β1整合素表达阳性细胞百分率（28．60±3．79）％均明显低于对照组，差异有统计学意义（P〈0．05）。结论β1整合素表达下调对结肠癌肝转移具有抑制作用。Objective To observe the inhibiting effect of down-regulation of integrin-β1 expression on hepatic metastasis of human colon carcinoma cell （HT-29）. Methods Integrin-β1 antisense oligodeoxynucleotide （ASODN） was transfected into HT-29 cell by liposome. The expressions of integrin-β1 mRNA and protein were determined by RT-PCR and Western-blot. The migration of HT-29 cells was assayed using transwell cell culture chambers in vitro. The models of hepatic metastasis in nude mice were established by the intrasplenic injection of transfected HT-29 cells. Thirty days later, the number and size of hepatic metastases were calculated, and the expression of integfin-β1 was studied by immunohistochemistry （SP）. Results In contrast with the control group,the expression of integrin-β1 mRNA and protein were significantly down-regulated in the experimental group （ P 〈 0.05 ） ; In vitro, the number of migrated HT-29 cells in the experimental group was far less than that of the control group （P 〈0.05 ） ;In vivo,the average number （4.00 ±0.93 ）/mouse,volume （ 10.10 ± 6.50） mm3/mouse ,weight （0. 0440 ~0. 0008） g/mouse and positive cell percentage （28.6 ± 3.79） % of hepatic metastases of the experimental group in nude mice were markedly decreased compared with the control group （ P 〈 0.05 ）. Conclusion Downregulation of integrin-β1 expression may play a key role in inhibiting hepatic metastasis of human colon carcinoma.

关 键 词：结肠癌 肝转移 Β1整合素 

分 类 号：R735.35[医药卫生—肿瘤] R619.6[医药卫生—临床医学]