改良大鼠杏仁核电点燃癫痫模型的建立  

Establishment of a modified amygdala kindling model of epilepsy in rats

作　　者：陈旭[1] 吴俊[1] 舒凯[1] 王建枝[2] 雷霆[1] 李龄[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经外科癫痫诊疗中心,武汉430030 [2]华中科技大学同济医学院病理生理学系

出　　处：《中华实验外科杂志》2009年第12期1726-1728,共3页Chinese Journal of Experimental Surgery

基　　金：国家863高技术研究发展计划资助项目（2006AA02241a）

摘　　要：目的建立一种改良的实用可靠的大鼠杏仁核电点燃癫痫模型。方法55只Wistar大鼠随机分成3组，其中空白对照组10只，手术对照组10只，刺激组35只。记录点燃过程中大鼠发作症状及脑电表现。点燃后灌注取脑，进行GFAP（胶原纤维酸性蛋白）免疫组化染色和TUNEL（原位DNA末端标记）染色，并与空白对照组和手术对照组进行比较。结果26只大鼠在（15．85±5．04）d成功点燃。点燃组海马区GFAP阳性细胞计数（67．26±4．52）较手术对照组（43．73±1．71）、空白组（46．51±3．83）显著增加（P〈0．05）。点燃组海马区TUNEL染色阳性细胞计数（26．03±2．57）较手术对照组（11．36±2．09）、空白组（10．28±2．34）显著升高（P〈0．05）。结论改良的杏仁核电点燃癫痫模型在症状学、电生理和神经病理等方面的改变与人类颞叶癫痫高度相似。Objective＇To establish a reliable,practical and modified amygdala kindling epilepsy model in rats. Methods Fifty-five male Wistar rats were randomly divided into 3 groups：10 in the blank control group,10 in the sham-operated group and 35 in the kindling group. The amygdala of the rats in the kindling group was stimulated with constant pulse current with frequency of 50 Hz, pulse duration of 1.0 ms, and train duration of 1 s for the initial 5 days and train duration of 10 s for the subsequent days. Rats＇ seizures and electroencephalogram were recorded throughout the stimulation process. TUNEL staining and immunohistochemical staining of GFAP were performed to evaluate the neuronal death and gliosis in the hippocampus of the kindled rats. Results Twenty-six rats were kindled successfully 15.85 ± 5.04 days after the kindling began. The expression of GFAP in the hippocampus of kindled rats （67.26 ± 4.52） was significantly stronger than that in the sham-operated group （43.73 ±1.71 ） and the blank control group （46.51 ± 3.83 ） （ P 〈 0.05 ）. TUNEL staining positive cells in the hippocampus of kindled rats （ 26.03 ±2.57） were significantly more than those in the sham-operated group （ 11.36 ±2.09） and the blank control group （ 10.28 ±2.34） （P 〈0.05）. Conclusion The modified amygdala kindling epilepsy model resembles the human temporal lobe epilepsy and is a favorable tool for the research of the mesial temporal lobe epilepsy.

关 键 词：癫痫 杏仁核 海马 模型 动物 

分 类 号：R742.1[医药卫生—神经病学与精神病学]