安徽铜陵狮子山硫化物矿山酸矿水中微生物功能群的研究  

An investigation into microbial function groups of acid mine drainage in the Shizishan sulfide mine,Tongling,Anhui Province

作　　者：孙青[1] 邢辉[1] 何斌[1] 李雪影[1] 陆现彩[2] 陆建军[2] 王睿勇[1] 

机构地区：[1]南京大学生命科学学院,江苏南京210093 [2]南京大学地球科学与工程学院,江苏南京210093

出　　处：《岩石矿物学杂志》2009年第6期547-552,共6页Acta Petrologica et Mineralogica

基　　金：国家重点基础研究发展计划资助项目(2007CB815603)

摘　　要：从安徽铜陵狮子山硫化物矿山酸矿水溪流获得样品,采用YE、Feo、FeSo、FeTo4种选择性培养基,利用overlay分离技术,获得7株细菌菌株。利用16S rRNA基因序列同源性分析,与目前已分离得到的酸矿水微生物进行了比较。利用分离菌株16S rRNA基因序列构建系统进化树,结果表明获得的菌株可分为3个功能群:嗜酸性异养菌、嗜酸性自养菌、中度嗜酸性铁氧化细菌。嗜酸性异养菌主要与酸矿水中三价铁的异化还原和寡营养状态的维持有关;嗜酸性自养菌与酸矿水中铁、硫元素的氧化有关,是酸水中的生产者;中度嗜酸性铁氧化细菌能将二价铁氧化成三价铁,并产生难溶性的矿物,可实现酸矿水与酸矿水底泥之间铁元素的动态平衡。Seven bacteria strains were isolated from the samples of an acid mine drainage stream in the Shizishan sulfide mine,Tongling,by using the overlay technique with four selective media of YE,Feo,FeSo and FeTo. The 16S rRNA gene sequences were amplified from total DNA of bacteria strains and were then sequenced and analyzed.The phylogenetic tree analysis with 16S rRNA gene sequences of isolated bacteria strains was carried out. The results show that every isolated bacteria strain can be divided into three microbial communities,namely heterotrophic acidophiles,autotrophic acidophiles and moderately acidophilic iron-oxidizing bacteria. The function of heterotrophic acidophiles lies in the dissimilatory ferric iron reduction and the creation of an oligotrophic environment. Autotrophic acidophiles can oxidize iron and sulfur elements in acid mine drainage,being producers of the acid mine drainage ecological system. Moderately acidophilic iron-oxidizing bacteria can oxidize ferrous iron and generate some insoluble minerals to realize the dynamic balance of iron element between the acid mine drainage and the sediments.

关 键 词：金属硫化物矿山 酸矿水 overlay分离技术 微生物功能群 系统进化树分析 

分 类 号：X141[环境科学与工程—环境科学] Q93-3[生物学—微生物学]