我国血液制品中人细小病毒B19污染情况及基因型的初步研究  

The prevalence and genotype of human parvovirus B19 in blood products

作　　者：吴瑜[1] 耿彦生[1] 王菁舟[1] 张勇朝[1] 赵晨燕[1] 孟淑芳[1] 李德富[1] 

机构地区：[1]中国药品生物制品检定所细胞室卫生部国家生物技术产品质量标准化重点实验室通,北京100050

出　　处：《中华微生物学和免疫学杂志》2009年第11期1031-1034,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家科技支撑计划（2008BAI54B00-4）

摘　　要：目的分析我国血液制品凝血因子Ⅷ及原料血浆中人B19病毒污染情况及B19病毒的基因型。方法以B19病毒VP1／VP2区引物采用套式PCR法分别检测16批凝血因子Ⅷ成品及10批混合原料血浆中B19病毒的污染情况，并对阳性样品中B19病毒DNA的NS1-VPlu区进行测序和基因型分析。结果16批凝血因子Ⅷ中B19病毒DNA阳性率为75％（12／16），10批混合原料血浆中B19病毒DNA阳性率为100％（10／10）。序列测定结果表明，所有B19阳性样品中的病毒序列高度保守，同源性为98．3％～100％，且均为基因型Ⅰ型。结论我国凝血因子Ⅷ及原料血浆中B19病毒Ⅰ型污染率高，病毒核苷酸具有较高的保守性。Objective To study the prevalence and genotype of human parvovirus B19 virus among blood products and plasma pools in China. Methods B19 DNA derived from 16 lots of factor Ⅷ concentrate produced by 4 manufactures and 10 lots of plasma pools were detected by nested PCR. Phylogenetic comparison of the partial B19 sequences obtained from positive samples were performed by direct sequencing. Results Twelve of sixteen lots of factor Ⅷ concentrate and all of ten lots of plasma pools were contaminated by B19 DNA. By comparing the partial B19 sequences, all the isolated viruses were genotype Ⅰ and their nucleotides were high conserved with homology of 98.3% -100%. Conclusion B19 genotype Ⅰ DNA has been detected in high prevalence in factor Ⅷ concentrate and plasma pools. The genetic diversities were shown to be very low.

关 键 词：人细小病毒B19 凝血因子Ⅷ 混合原料血浆 基因型 

分 类 号：R[医药卫生]