雌激素受体alpha对大鼠骨髓基质细胞诱导分化成脂的调控作用  

Role of estrogen receptor alpha in adipocytes differentiation of rat bone marrow mesenchymal stem cells

作　　者：王婧[1] 丁寅[1] 

机构地区：[1]西安第四军医大学口腔医学院正畸科,710032

出　　处：《实用口腔医学杂志》2009年第6期794-797,共4页Journal of Practical Stomatology

摘　　要：目的:研究雌激素受体-α(ER-α)与脂肪代谢的相关性。方法:培养大鼠骨髓基质细胞(rBMSC)并传代,将第3代rBMSC随机分为3组,一组为对照组,一组用脂质体转染法转入体外构建的ER-α高表达的重组质粒,一组加入10-6mol/L的雌激素受体的抑制剂枸橼酸他莫西芬(Tamoxifen citrate),Western blot检测转染前后及加入抑制剂前后2组细胞中ER-α的表达变化;对以上3组细胞进行成脂肪诱导;10 d后对3组细胞进行油红O染色,计算红色脂滴的数量,通过多个样本均数的方差分析比较3组之间的差别。结果:Western blot检测到转染后,ER-α的条带表达增强;加入抑制剂后,ER-α的条带表达减弱。3组不同处理的细胞形成脂滴数量的差异有显著统计学意义,P值均<0.01。3组细胞形成脂滴的数量为:ER-α低表达组>对照组>ER-α高表达组。结论:大鼠骨髓基质细胞成脂肪分化过程中,ER-α的表达影响脂肪细胞的形成与分化,推测ER-α抑制脂肪细胞形成。Objective：To elucidate the mechanism of the role of ER-α in fat metabolism by regulating the expression of ER-α in SD rats marrow mesenchymal stem cells during differentiating into adipoeytes in vitro. Methods：SD rats marrow mesenchymal stem cells were separated and cultivated. The ER-α was transfected into the rBMSCs in group one. Tamoxifen Citrate was used to restrain the expression of ER-α in group two, the group without any treatment was used as control. Western blot was used to identify the difference of ER-α expression among different groups and Oil-Red-O staining was employed to identify the adipocytes in vitro. Results ： There was significant difference between the number of lipids and different groups （ P 〈 0.01 ）, the number of lipid droplet changed concomitantly with ER-α： Low-expression ER-α group 〉 Control group 〉 High-expression ER-α group. Conclusion： ER-α in SD rats marrow mesenchymal stem ceils may restrain the adipocyte differentiation.

关 键 词：骨髓基质细胞 雌激素受体-Α 成脂诱导 转染 枸橼酸他莫西芬 

分 类 号：R78[医药卫生—口腔医学]