短期高浓度氟对小鼠磨牙胚中牙本质涎蛋白表达的影响  

Effect of short exposure to high concentrations of fluoride on the expression of DSP in odontoblasts of mice molars

作　　者：张菁[1] 冀章章[1] 梅陵宣[1] 

机构地区：[1]安徽医科大学附属口腔医院口腔内科,230032

出　　处：《实用口腔医学杂志》2009年第6期816-819,共4页Journal of Practical Stomatology

摘　　要：目的:研究短期高浓度氟对小鼠磨牙成牙本质细胞形态及牙本质涎蛋白(dentin sialoprotein,DSP)表达的影响,探讨氟对牙本质发育的作用机制。方法:选择4 d龄的ICR小鼠共32只,随机分为2组,每组16只,各组中实验动物和对照动物各半。实验动物单次腹腔注射剂量分别为10 mg/kg体重和20 mg/kg体重的NaF,对照动物单次腹腔注射等剂量的NaCl,注射量均为10μl/g,24 h后处死动物。采用HE染色、免疫组化染色观察高浓度氟对小鼠磨牙不同分化阶段成牙本质细胞形态及DSP的表达,采用SPSS 13.0软件对数据进行分析。结果:实验组分泌期成牙本质细胞形态紊乱,正常的高柱状形态丧失,DSP的表达明显强于对照动物,差异有统计学意义(P<0.01),而成熟期成牙本质细胞未见明显变化。结论:短期高浓度氟能增强分泌期成牙本质细胞中DSP的表达,抑制成牙本质细胞的增殖分化及随后的基质合成与分泌,从而影响牙本质的发育。Objective： To study the effect of short exposure to high levels of fluoride on cellular structure and the DSP expression of odontoblasts of mice molars. Methods： 32 4-day-old ICR mice were randomly divided into two groups. The experimental animals were received a single intraperitoneal close of 20 mg（ n = 8） and 10（ n = 8） mg NaF/kg（ body weight） respectively. Equal doses of NaCl were given to the controls （ n = 8 for each group）. The injected volume was kept constant （ 10 μl/g）. After 24 hours all mice were sacrificed. HE staining and immunohistochemical staining were used to observe the structural changes and the expression of DSP in odontoblasts of mouse molar at different stages. SPSS 13.0 software package was used to analyze the results. Results： The secretory odontoblasts distorted and lost its normal column contour. The immunohistochemistry resuhs demonstrated that the expression of DSP was more intense than that in control group. Results of statistics analysis showed there were significant differences between the two groups（ P 〈 0.01 ）. No remarkable differences were found in mature odontoblasts. Conclusion： Short exposure to high concentration of fluoride can enhanee the expression of DSP in the secretory odontoblasts, and inhibit differentiation of the odontoblasts and matrix secretion. This lead to the abnormal development of dentinogenesis.

关 键 词：氟化物 成牙本质细胞 牙本质涎蛋白 

分 类 号：R78[医药卫生—口腔医学]