加热联合黄体酮对人舌癌耐药细胞株Tca8113/BLM耐药性的影响  

Effect of hyperthermia in combination with progresterone on a human tongue cancer drug-resistance cell line Tca8113/BLM

作　　者：何春梅[1] 秦迪兰[1] 邓珂[1] 

机构地区：[1]湖南大学生物医学工程中心生物纳米与分子工程实验室,410082

出　　处：《实用口腔医学杂志》2009年第6期862-864,共3页Journal of Practical Stomatology

基　　金：湖南省卫生厅(编号:B2003-115)

摘　　要：目的:研究加热(hyperthermia,HT)联合黄体酮(progresterone,Prog)对人舌癌细胞Tca8113和耐药细胞Tca8113/BLM耐药性的影响。方法:采用MTT法检测加热41℃1 h后联合Prog对人舌癌亲本细胞Tca8113和耐药细胞Tca8113/BLM化疗药物的敏感性;流式细胞仪(FCM)检测细胞内阿霉素(ADM)浓度聚积以及细胞膜上P-gp和MRP的表达。结果:与黄体酮作用细胞相比,加热41℃1 h联合2μmol/L Prog作用细胞后,Tca8113和Tca8113/BLM细胞的IC50显著下降(P<0.01),且各组之间均有显著差异(P<0.01),ADM浓度聚积在2种细胞中明显增加(P<0.01),细胞膜上的P-gp和MPR荧光强度显著下降(P<0.01)。结论:加热联合Prog能协同增加细胞内药物浓度的聚积,显著提高细胞对化疗药物BLM的敏感性,其二者协同作用与降低细胞膜上的P-gp和MRP的表达有关。Objective： To explore the possible effect of hyperthermia combined with Prog on a human tongue cancer cell line TcaSll3 and drug-resistance cell line Tca8113/BLM in vitro. Methods ：The effect of hyperthermia combined with Prog on Tca8113 and Tea8113/ BLM cells were determined by MTT. The concentration of ADM, the expression of P-gp and MRP were detected by flow cytometry （FCM）. Results：Compared with Prog or hyperthermia, the hyperthermia at 41 ℃ for 1 h combined with Prog showed obvious inhibitory effect in Tca8113 and Tca8113/BLM cells（ P 〈0.01 ）. The concentration of ADM in Tca8113 and Tca8113/BLM cells increased obviously （ P 〈 0.01 ）. Expression levels of P- gp and MRP in Tca8113 and Tca8113/BLM cells decreased significantly. Conclusion ： The hyperthermia combined with Prog can increase the intracellular concentration of ADM in Tca8113 and Tca8113 /BLM cells, which reverse the drug-resistance of Tca8113/BLM cells to BLM and down-regulate expressions of P-gp and MRP.

关 键 词：加热 黄体酮 Tca8113/BLM P—gp MRP 

分 类 号：R739.8[医药卫生—肿瘤]