妊娠及分娩后TA2小鼠T细胞亚群及IL-2水平变化在自发乳腺癌中的作用  

The Effect of the Changes in T Lymphocyte Subsets and IL-2 During Different Stages of Pregnancy and Puerperium on the Carcinogenesis of TA2 Mice

作　　者：王璇[1] 谷彦军[2] 黄纯[3] 李岩[2] 崔艳芬[2] 徐少艳[2] 张诗武[2] 孙保存[4] 

机构地区：[1]天津医科大学营养与食品卫生学教研室 [2]乳腺癌防治教育部重点实验室天津市肿瘤防治重点实验室天津医科大学附属肿瘤医院肿瘤研究所分子病理室,天津市300060 [3]天津医科大学附属肿瘤医院肺部肿瘤内科 [4]天津医科大学病理学教研室

出　　处：《中国肿瘤临床》2009年第22期1310-1314,共5页Chinese Journal of Clinical Oncology

基　　金：天津市科委重大科技攻关基金(编号:043115211-1)

摘　　要：目的:观察自发乳腺癌和妊娠及分娩后不同时间,TA2小鼠T细胞亚群及IL-2水平的变化,探讨妊娠及分娩在TA2小鼠自发乳腺癌中可能发挥的作用。方法:将TA2小鼠分为正常未妊娠组、自发瘤组、妊娠5、10、20天组和分娩后1、7、14、21天共9组,采用流式细胞术、ELISA等方法,测定小鼠脾脏CD3+、CD4+、CD8+T细胞亚群,计算CD4/CD8,并测定血清IL-2水平。结果:与正常未妊娠组比较,TA2小鼠妊娠及分娩后不同时间内CD3+T细胞亚群百分比均有下降(P<0.01),在妊娠10天下降至最低(P<0.01),CD4+细胞百分比、CD4+/CD8+在妊娠20天降至最低(P<0.01;P<0.05),而CD8+细胞百分比升至最高(P<0.01)。与自发瘤组比较,除妊娠10天CD3+T细胞亚群百分比与荷瘤小鼠无明显差别外,其他各组均高于自发瘤组(P<0.05或P<0.01);妊娠期各组CD4+、CD8+、CD4/CD8均与自发瘤组无差别。IL-2在妊娠期间有所下降,以10天和20天组下降显著(P<0.05),分娩后1天恢复至正常未妊娠时水平,但在分娩后7、14、21天又逐渐下降,在分娩后21天明显低于正常未妊娠组(P<0.01),甚至与自发瘤组接近(P<0.01)。结论:妊娠和分娩在一定程度上会影响TA2小鼠T细胞亚群分布和IL-2水平的变化,使其细胞免疫功能受抑制,成为TA2小鼠自发性乳腺癌发生的可能诱因之一。Objective： To observe the changes in T lymphocyte subsets and IL-2 during different stages of pregnancy and puerperium in TA2 mice, and to investigate the possible influences of pregnancy and puerperium on the cellular immune function and the carcinogenesis of TA2 mice. Methods： The TA2 mice were divided into 9 groups including normal group, spontaneous breast cancer group, mice on the 5th day of pregnancy, 10th day of gestation, 20th day of gestation and mice on the 1st, 7th, 14th, and 21st days of postpartum. The percentages of CD3＋, CD4＋ and CD8＋ T cell subsets in mice spleen were examined by flow cytometry. The ratio of CD4＋ to CD8＋ T cell subsets （CD4/CD8） was caculated, and IL-2 in mice serum was determined by ELISA. Results： Compared with that in the normal group, the percentage of CD3 ＋ T cell subsets was de- creased during every stage of pregnancy and postpartum （P〈0.01） and reached the lowest level on the 10th day of pregnancy （P〈0.01）. The percentages of CD4＋ T cell subsets and CD4/CD8 were decreased to the lowest in 20th day of pregnancy group （P〈0.01 and 〈0.05）, while the percentage of CD8＋ T cell subsets was increased to the highest （P〈0.01）. Compared with that in the spontaneous breast cancer group, the percentage of CD3＋ T cell subsets in other groups （except in the 10th days of pregnancy group） was higher. There was no significant difference in the percentages of CD4＋ and CD8＋T cell subsets and CD4/CD8 ratio between the spontaneous breast cancer group and other groups. IL-2 level was decreased during pregnancy, and there was a significant difference between the 10th day of pregnancy group and the 20th day of pregnan- cy group （P〈0.05）. Although IL-2 was increased on the 1st day after delivery, it was decreased gradually in the 7th, 14th, and 21st days of postpartum groups, significantly lower than that in the normal group （P〈0.01） and similar to that of the spontaneous breast cancer group （P〈0.01）. Conclusion： Pregn

关 键 词：TA2 自发性乳腺癌 妊娠 T细胞亚群 IL-2 

分 类 号：R737.9[医药卫生—肿瘤]