COX-2抑制剂塞来昔布对人舌鳞癌Tca8113细胞迁移的抑制作用  

Inhibitory effects of COX-2 inhibitor on migration of human tongue squamous cell carcinoma Tca8113 cells

作　　者：霍秋菊[1] 李伟忠[1] 

机构地区：[1]南方医科大学南方医院口腔科,广东广州510515

出　　处：《南方医科大学学报》2009年第11期2194-2196,共3页Journal of Southern Medical University

基　　金：广东省自然科学基金(06024396)

摘　　要：目的研究环氧化酶-2(COX-2)抑制剂塞来昔布(Celecoxib)抑制人舌鳞癌Tca8113细胞迁移的作用。方法采用细胞划痕实验、细胞-基质黏附实验和Boyden趋化小室测定Tca8113细胞的迁移能力,观察塞来昔布对Tca8113细胞迁移作用的影响。结果10μmol/L和20μmol/L塞来昔布处理细胞24h后,细胞迁移数与对照组相比减少有统计学意义(P<0.05),Tca8113细胞的迁移能力显著减弱。不同浓度塞来昔布处理Tca8113细胞24h后,其与包被Fn基质胶表面上的黏附情况呈剂量依赖关系,随塞来昔布浓度的增加,Tca8113细胞在基质表面的黏附显著降低。结论COX-2抑制剂塞来昔布具有抑制Tca8113细胞迁移的能力,其机制有待进一步研究。Objective To investigate the role of COX-2 inhibitor celecoxib in inhibiting the migration of human tongue squamous cell carcinoma Tca8113 cells.Methods The effects of celecoxib on Tca8113 cell migration were tested using scrape motility assay,cell-matrix adhesion assay and Boyden chamber motility assay.Results Following a 24-hour incubation with 10 and 20 μmol/L celecoxib,the migration of Tca8113 cells was significantly decreased（P〈0.05）.Celecoxib treatment for 24 h also resulted in significantly decreased adhesion of Tca8113 cells on Fn-coated surface in a dose-dependent manner.ConclusionCOX-2 inhibitorcelecoxib can inhibitTca8113 cellmigration,the mechanismofwhich awaitsfurtherinvestigation.

关 键 词：环氧化酶-2 塞来昔布 癌 鳞状细胞 舌肿瘤 细胞迁移 

分 类 号：R739.8[医药卫生—肿瘤]