Tiam1在乳腺癌组织中的表达及临床意义  

Expression of Tiam1 in breast carcinomas and its clinical significance

作　　者：吴元清[1] 彭刚[1] 

机构地区：[1]湘潭职业技术学院护理系,湖南湘潭411100

出　　处：《南方医科大学学报》2009年第11期2324-2326,2328,共4页Journal of Southern Medical University

摘　　要：目的探讨T淋巴瘤侵袭转移诱导因子1(Tiam1)蛋白在乳腺癌组织中的表达,并探讨其与乳腺癌临床病理特征之间的关系。方法采用免疫组织化学检测126例临床乳腺癌组织标本中Tiam1的表达情况。应用RT-PCR和Western blotting检测不同转移潜能乳腺癌细胞株中Tiam1 mRNA和蛋白的表达情况。结果在乳腺癌组织Tiam1表达明显高于良性乳腺组织(P<0.05)。Tiam1的表达与患者年龄、组织学分型无相关性(P>0.05),与淋巴结转移和临床分期显著相关(P<0.01)。应用RT-PCR和Western blotting检测显示Tiam1 mRNA和蛋白在高转移潜能的乳腺癌细胞株中相对高表达。结论Tiam1和乳腺癌转移行为密切相关,可能在促进乳腺癌转移方面发挥重要作用。Objective To investigate the expression of T lymphoma invasion and metastasis inducing factor 1（Tiam1） in breast carcinomas,and explore its association with the clinicopathological features of breast carcinoma.Methods Immunohistochemistry was used to detect Tiam1 expression in normal breast tissue and 126 breast carcinoma tissues,and the expression levels of Tiam1 mRNA and protein were detected by reverse transcriptase polymerase chain reaction（RT-PCR） and Western blotting,respectively.Results The expression of Tiam1 was significantly higher in breast carcinomas than in normal breast tissue（P〈0.05）.Tiam1 expression was not correlated to the age of the patients or the histological type（P〉0.05）,but to lymph node metastasis and clinical stages of the tumor（P〈0.01）.Tiam1 mRNA and protein expressions were stronger in breast carcinoma cell line MDA-MB-435 with high metastatic potential than in breast carcinoma cell line MCF-7.Conclusion Tiam1 is closely related to the metastasis of breast carcinoma,and may play an important role in promoting metastasis of breast carcinoma.

关 键 词：乳腺肿瘤 T淋巴瘤侵袭转移诱导因子1 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤]