用^(125)Ⅰ标记单抗检测非吸烟女性肺癌血浆中ras癌基因产物p21蛋白  

Determination of Iodine^(125) labelled monoclonal ras oncoprotein in plasma from non smoking female lung cancer patients and healthy controls

作　　者：朱莉芳[1] 程家蓉[1] 李宏年[1] 钟礼杰[1] 瞿永华[1] 

机构地区：[1]上海市肿瘤研究所,200032

出　　处：《肺癌杂志》1998年第1期37-39,共3页

摘　　要：目的　探讨肺癌发生与癌基因表达产物之间的相互关系。方法　用 (SDS PAGE)WesternBlotting方法结合单克隆抗体的同位素标记技术 ,检测上海市非吸烟女性肺癌患者和非吸烟健康女性对照人群血浆中ras癌基因表达产物p2 1蛋白。结果　肺癌人群 3 8例中 3 5例p2 1蛋白为阳性 ,对照人群59例中 1 7例为阳性。两者相对危险度估计值 (OR)为 2 8.8(χ2 MH=3 6.85)。 3 5例阳性中 2 3例为“ +” ,8例为“ ” ,4例为“ ”。它们的OR分别为 2 1 .5、1 1 2 .0、56.0 (χ2 MH分别是 2 7.45、3 0 .58、1 9.70 )。肺癌人群p2 1表达阳性率明显高于健康人群 ,有非常显著意义 (P <0 .0 0 1 )。结论　本方法有可能作为分子流行病学调查的一个生物学标志 ,并可能有助于临床诊断 ,以及用于环境、职业性高暴露人群及高危个体的健康监护。Objective To explore the significance of oncoprotein in plasma in the carcinogenic process of lung cancer. Methods ras oncoprotein in plasma was assayed in 38 lung cancer patients and 59 healthy women for controls among non smoking females by Western Blot analysis with monoclonal ras antibody as the primary antibody and iodine 125 labelled technique.Results Positive samples were found in 35 of the 38 patients versus 17 of the 59 controls, odds ratio(OR) was 28.8(χ 2 MH =36.85). OR for 35 positive samples with 23(+),8(),4() was 21.5 , 112.0 and 56.0 respectively (χ 2 MH =27.45,30.58 and 19.70). ras oncoprotein levels of the plasma from the lung cancer patients were statistically significantly higher than those from the controls(P< 0.001 ). Conclusion An increasing of ras oncoprotein in plasma could be a possible biomarker of epidemiologic study for female lung cancer. ras oncoprotein may be used to health surveillance of high risk population due to environmental and occupational exposure.

关 键 词：肺癌 RAS基因 P21蛋白 女性 非吸烟 碘125 

分 类 号：R734.202[医药卫生—肿瘤]