载脂蛋白A-I启动子调控的肝靶向性载体的构建与鉴定  

Construction and identification of liver-specific vector utilizing the apolipoprotein A-I promoter

作　　者：任雪玲[1] 徐敏[1] 张楠[1] 张振中[1] 张云[1] 

机构地区：[1]郑州大学药学院药物分析系,河南郑州450001

出　　处：《中国现代医学杂志》2009年第22期3403-3406,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金资助项目(No:30772543)

摘　　要：目的构建载脂蛋白A-I启动子调控的肝特异性真核表达载体。方法PCR扩增载脂蛋白A-I组织特异性启动子序列,将其亚克隆至绿色荧光真核表达载体中。将重组载体以及对照载体转染肝癌细胞株SMMC-7221和肺癌细胞株A549。结果载脂蛋白A-I启动子调控的绿色荧光蛋白在肝癌细胞中表达,而在肺癌细胞中没有表达。CMV启动子调控的绿色荧光蛋白在两种细胞株中都呈现高效表达。结论载脂蛋白A-I启动子调控的绿色荧光蛋白可以进行组织特异性表达,成功构建具有肝靶向性的真核表达载体。[ Objective ] A liver-specific targeting vector for hepatoma gene therapy was studied using the human apolipoprotein A-I peomoter. [ Methods ] The apoA-I promoter containing essential transcriptional regulatory elements was amplified and then inserted into upstream of the green fluorescent protein gene of pEGFP. The recombinant plasmid with apoA-I promoter and the control plasmid with CMV promoter were transfected into SMMC-7221 cell and A549 cell. [ Results ] Cells generated using vector with liver-specific promoter expressed GFP only in the appropriate cell type. By contrast, when transfected with the control vector, cells gave similar levels of expression. [ Conclusions ] We developed the recombinant vector, carrying the fluorescent protein gene under the control of the apoA-I promoter to obtain restricted expression of a exogenous gene only in liver cells.

关 键 词：apoA-I启动子 肝癌 绿色荧光蛋白 基因治疗 

分 类 号：R735.5[医药卫生—肿瘤] R730.5[医药卫生—临床医学]