饮食、运动对胰岛素抵抗大鼠骨骼肌中GSK-3表达的影响  

Effects of diet and exercise on the expression of glycogen synthase kinase-3 in skeletal muscle of rats with insulin resistance

作　　者：陈丹[1] 毕会民[1] 

机构地区：[1]湖北省新华医院内分泌科,湖北武汉430015

出　　处：《中国现代医学杂志》2009年第22期3421-3424,共4页China Journal of Modern Medicine

摘　　要：目的观察饮食控制和运动锻炼对高脂高糖饮食诱导的胰岛素抵抗模型大鼠骨骼肌中,糖原合酶激酶-3表达的影响。方法将40只Wistar大鼠随机分为正常组、模型组、饮食组和运动组,每组10只。模型组、饮食组和运动组均喂以高糖高脂饲料。4周后饮食组大鼠改以常规饲料喂养,运动组则在继续高糖高脂饲料基础上加以每周游泳5d,连续6周。实验结束后,采用Western blot法检测各组大鼠骨骼肌中GSK-3的含量,定期检测其体质量、血甘油三脂和胆固醇、空腹血糖和胰岛素水平,并计算胰岛素敏感指数。结果模型组大鼠骨骼肌中GSK-3的含量较正常组增高70.20%(P<0.01);饮食控制6周后,大鼠骨骼肌中GSK-3的含量较模型组减少23.22%(P<0.01);运动锻炼6周后,大鼠骨骼肌中GSK-3的含量较模型组减少27.24%(P<0.01)。结论饮食控制和运动锻炼均可降低胰岛素抵抗大鼠骨骼肌细胞GSK-3蛋白表达水平,从而加强葡萄糖的摄取和利用,改善胰岛素抵抗。[ Objective ] To observe the effects of diet and exercise on the expression of GSK-3 in skeletal muscle of rats with insulin resistance induced by high-fat and sucrose diets. [ Methods ] 40 Wistar rats were divided into four groups medially and randomly： control group, model group, diet group and exercise group. The model group, diet group and exercise group were given the high-fat and sucrose diets （20% sucrose, 10% lard, 2.5% cholesterol, 1% cholic acid） for 4 weeks. Then diet group were fed with normal diets and exercise group were trained by swimming regularly for 6 weeks. At the end of the experiment, GSK-3 protein was detected by Western blot analysis. Body weight, serum triglyceride and cholesterol, fasting plasma glucose and serum insulin concentration were measured timely and insulin sensitivity index was computed as well. [ Results ] The outcome of Western blot showed that the expression of GSK-3 in skeletal muscle of model group rats increased by 70.20% （P 〈0.01） compared with control group rats. After 6 weeks of diet-control or exercise training, the GSK-3 protein in skeletal muscle of diet group and exercise group rats decreased by 23.22% （P 〈0.01） and 27.24% （P 〈0.01） respectively compared with model group rats. [ Conclusion] Not only diet control but also exercise training can lower the expression of GSK-3 in skeletal muscle of insulin resistance rats, consequently promoting the uptake and utilize of glucose, to improve the insulin resistance.

关 键 词：饮食 运动 糖原合成酶激酶-3 胰岛素抵抗 动物模型 

分 类 号：Q53[生物学—生物化学] R576[医药卫生—消化系统]