粘放菌5519Ⅰ型菌毛兔抗血清的分离及鉴定  

Separation and identification of antibodies against type Ⅰ fimbriae of Actinomyces viscosus 5519.

作　　者：梁景平[1] 朱敏[1] 黄冬生 刘正[1] 

机构地区：[1]上海第二医科大学口腔医学院口腔内科

出　　处：《广东牙病防治》1998年第4期1-2,共2页Journal of Dental Prevention and Treatment

基　　金：国家自然科学基金

摘　　要：本实验在纯化粘性放线菌５５１９Ⅰ型菌毛的基础上，以粘放菌Ｉ型菌毛蛋白作为抗原，采用多途径免疫方法进行了兔抗Ｉ型菌毛抗血清的分离和提取，并以免疫扩散和琼脂凝胶免疫电泳进行了鉴定。实验结果显示，粘放菌５５１９Ｉ型菌毛蛋白的分子量大部分为５４ＫＤ，少量为３８ＫＤ，抗体效价可达１：３２，且与粘放菌５９５１Ⅱ型菌毛无交叉反应出现。On the basis of purifying type Ⅰ Actinomyces viscosus 5519, type Ⅰ fimbriae were used for antigens. Separation and extraction of polyclonal antibodies against the type Ⅰ fimbriae of Actinomyces viscosus were carried out through immunity of poly ways. Antibodies were tested by double immunodiffusion and immunoelectrophoresis. These findings showed that most of the molecular mass of type Ⅰ fimbriae is 54KD, small parts is 38KD. Concentration of antibodies against type Ⅰ fibriae reached 1:32, and had no cross reaction with type Ⅱ fibriae of Actinomyces viscosus5951.

关 键 词：粘性放线菌 I型菌毛 抗血清 分离 鉴定 

分 类 号：R780.2[医药卫生—口腔医学] R379.1[医药卫生—临床医学]