高效液相色谱法检测化妆品中儿茶酚胺类药物的残留量  

Determination residual catecholamines in cosmetics by HPLC

作　　者：吴延晖[1] 许泓[1] 肖亚兵[1] 何佳[1] 张骏[1] 林安清[1] 张曼[1] 

机构地区：[1]天津出入境检验检疫局,天津300201

出　　处：《日用化学工业》2009年第6期439-441,共3页China Surfactant Detergent & Cosmetics

摘　　要：采用高效液相色谱法,研究检测了化妆品中肾上腺素、去甲肾上腺素、异丙肾上腺素的残留量。得到的色谱条件:W aters Atlantis C18柱(5μm,250 mm×4.60 mm)为分析柱,流动相V(甲醇)∶V(0.02 mol/L磷酸二氢钾溶液)=4∶96,荧光检测器检测,激发波长为285 nm,发射波长为340 nm。结果表明,肾上腺素、去甲肾上腺素在0.025 mg/L^2.5 mg/L内,异丙肾上腺素在0.05 mg/L^5.0 mg/L内,其峰面积与质量浓度呈良好的线性关系。该方法回收率为95.6%~99.4%,RSD为3.6%~6.1%(n=8)。A HPLC method for the determination the residual of catecholamines including epinephrine, norepinephrine and isoprenaline in cosmetics was established. The extract was analyzed on Waters Atlantis C18 column （ 5 um, 250 mm × 4. 60 mm ） with mobile phase consisted of V （ methanol ） ： V （ 0. 02 mol/L monopotassium phosphate solution ） = 4： 96. The detection was conducted by fluorescence detector with excitation wavelength of 285 nm and emission wavelength of 340 nm. Results showed that as the concentration of epinephrine, norepinephrine is in the range of 0. 025 mg/L - 2. 5 mg/L, and the concentration of isoprenaline is in the range of 0. 05 mg/L- 5.0 mg/L, the relationship between concentration and the corresponding peak area shows good linearity. The recovery achieves 95.6%- 99.4%. The RSD of the method is 3.6% -6. 1% （n =8）.

关 键 词：化妆品 肾上腺素 去甲肾上腺素 异丙肾上腺素 高效液相色谱法 

分 类 号：TQ658[化学工程—精细化工]