曲古抑菌素A对乳腺癌细胞株MDA-MB-231增殖的影响  

Effect of Trichostatin A on cell proliferation of breast cancer cell line MDA-MB-231

作　　者：聂建云[1] 刘馨[1] 黄云超[1] 

机构地区：[1]昆明医学院第三附属医院,昆明650118

出　　处：《山东医药》2009年第43期22-23,共2页Shandong Medical Journal

基　　金：中国人事部留学人员科技活动项目择优资助启动基金;云南省科技厅应用基础研究项目(No2007C0026R)

摘　　要：目的探讨曲古抑菌素A(TSA)对乳腺癌细胞株增殖的影响。方法培养乳腺癌细胞株MDA-MB-231,用组不同浓度TSA分别作用于细胞,于24、48、72、96 h用MTT比色法观察细胞生长情况,流式细胞术检测S期细胞的比例和周期素(Cyclin)D1、A2表达。结果72h内和4μmol/L的浓度范围内,乳腺癌细胞生长受到抑制,并有一定的时效关系;TSA作用后周期素Cyclin A2表达下降,Cyclin D1表达上升(P均<0.05)。结论TSA能抑制乳腺癌细胞生长,周期素Cyclin D1、Cyclin A2参与了抑制剂对细胞增殖周期的调控。Objective To investigate the effect of Trichostatin A （ TSA ） on the cell proliferation of breast cancer cell line. Methods The breast cancer cell line MDA-MB-231 was cultured and treated with TSA in different concentrations. The cell growth was detected by M＇Iq＂ assay 24,48 ,72 and 96 hours later,the proportion of S-phase cell and expression of Cyclin A2,Cyclin D1 were measured by the flow eytometry. Results The cell growth was inhibited in time-effect manner 24-72 hours after the treatment of TSA in concentration of 1 - 4 lxInol/L. The expression of Cyclin A2 decreased, the expression of Cyelin D1 increased after the treatment of TSA. Conclusions TSA can inhibit the cell growth of breast cancer , Cyclin A2 and Cyelin D1 involes in the regulation of cell proliferation cycle.

关 键 词：乳腺癌 曲古抑菌素A 细胞增殖 

分 类 号：R737.9[医药卫生—肿瘤]