气相色谱内标法测定蚕蛹油中α-亚麻酸含量  

Content Determination of α-Linolenic Acid in Can Yong Oil by GC Internal Standard Method

作　　者：路萍[1] 赖炳森[1] 颜小林[1] 王映强[1] 郑成贵[1] 谭亚芳[1] 

机构地区：[1]解放军北京医学高等专科学校,北京100071

出　　处：《药物分析杂志》1998年第6期390-393,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：化学法提取的蚕蛹油经甲酯化后 ,采用气相色谱 /质谱 (GC/MS)技术 ,对蚕蛹油中脂肪酸的组分进行了分析 ,以十九烷酸 (19∶0 )甲酯作内标物 ,气相色谱法 (GC)对蚕蛹油中α -亚麻酸进行了定量测定。结果表明蚕蛹油中α -亚麻酸的含量高达 72 80 %。本研究为αCan Yong oil was extracted by chemical method.The fatty acid(FA)in Can Yong oil was saponificated by KOH-MeOH and was modified to methyl esters by means of BF 3-MeOH,and was checked with thin-layer chromatography.The compositions of fatty acids were analyzed by GC/MS.The method of GC internal quantification of α-Linolenic in Can Yong was established,using internal standard of C 19∶0 .A linear relationship was obtained in the concentration rage of 0 125～2 000 μg/μL and the correlation coefficient was 0 9830.The content of α-Linolenic acid was 72 80 %.The study may give a test method for determination of α-Linolenic.

关 键 词：气相色谱 Α-亚麻酸 蚕蛹 

分 类 号：R931.74[医药卫生—生药学] R927.2[医药卫生—药学]