兔死后肝细胞自溶的超微结构观察  

Ultra-structure changes of liver autolysis

作　　者：秦志强[1] 龚毓昌[1] 俞永富[2] 

机构地区：[1]司法部司法鉴定科学技术研究所,上海200063 [2]上海医科大学,上海200032

出　　处：《法医学杂志》1998年第4期207-208,共2页Journal of Forensic Medicine

摘　　要：用透射电镜观察兔死后肝细胞的超微结构变化，发现：在0．5h即有轻微的自溶改变，表现为细胞核中异染色质异常凝集和滑面内质同扩张；在1h自溶变化已趋明显，且随死后时间延长，在2h、3h、4h、5h该变化越来越广泛而显著，可见线粒体肿胀，嵴断裂、消失，基质内絮状致密体（Flocculentdensehadies，FDB）形成，粗面内质网及核周池扩张等改变。肝细胞自溶的超微结构变化与死后时间相关，这对推断早期死亡时间有应用价值。Ultra - stucture changes of autolysis of hepatocyte werestudied using transmission electron microscope(TEM). It wasfound that autolysis Started 0. 5 hour after death, with theheterochromatin of nuclei shrinking abnormally and smooth endoplasmic reticulum expanding. The changes developed todistinction after 1h, and progressed continuously after 2h,3h, 4h and 5h. It was found that (1) the mitochondria wereswelling, its matrix density decreasing and flocculent densebodies (FDB) emerging; (2) the rough endoplasmic reticulum and perinuclear cistern were expanding. It was suggestedthat ultra - structure changes of the autolysis be correlated tothe postmortem time.

关 键 词：肝细胞 自溶 超微结构 死亡时间 

分 类 号：D919.4[医药卫生—法医学]