鱼体中硝基苯残留的气相色谱-质谱法测定  

Determination of Nitrobenzene Residue in Fish Using Gas Chromatography and Mass Spectrometry

作　　者：魏志强 冷凯良[2] 孙伟红[2] 王志杰[2] 战培荣[3] 陈忠祥[3] 

机构地区：[1]山东省青岛市环境监测中心站,山东青岛266003 [2]中国水产科学研究院黄海水产研究所,山东青岛266071 [3]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070

出　　处：《安徽农业科学》2009年第35期17299-17300,17305,共3页Journal of Anhui Agricultural Sciences

基　　金：农业部标准化项目

摘　　要：[目的]研究鱼体中硝基苯残留的测定方法。[方法]样品经丙酮提取、水蒸气蒸馏净化、苯反萃取,采用气相色谱-质谱法测定。[结果]硝基苯的峰面积与质量浓度在5～1 000 ng/ml范围内呈线性关系,相关系数>0.999,回收率为75%～110%,相对标准偏差为4.64%～6.13%,最低检测限为3.0μg/kg。[结论]该研究建立了鱼体中硝基苯残留的气相色谱-质谱法,方法简便、快速、准确。[ Objective ] A method for determining residue of uitrobenzene in fish was studied. [ Method ] The sample was extracted with acetone, cleaned-up with water vapour, and extracted by benzene. The mass spectrometer was operated for qualitative and quantitative analysis of nitrobenzene. [ Result ] The correlation coefficient of linear calibration curve was over 0. 999 within the nitrobenzene concentration range 5 - 1 000 ng/ml. The recovery rate for nitrobenzene was between 75% and 110%. The relative standard deviation was between 4.64% and 6.13% and the limit of detection was 3.0 μg/kg for GC-MS. [ Conclusion] A simple, rapid and high accuracy method for determining residue of uitrobenzene in fish was established using gas chromatography and mass spectrometry.

关 键 词：硝基苯 气相色谱-质谱仪(GC—MS) 鱼体 

分 类 号：O657.71[理学—分析化学]