欧李茎尖脱毒快繁研究  

Study on the Virus-free and Rapid propagation of Prunus humilis(Bunge.)

作　　者：王正德[1] 庞发虎[1] 

机构地区：[1]南阳师范学院生物系,河南南阳473000

出　　处：《安徽农业科学》2009年第35期17797-17798,共2页Journal of Anhui Agricultural Sciences

基　　金：南阳师范学院资助项目

摘　　要：[目的]研究欧李脱毒快繁技术。[方法]以欧李嫩梢芽为外植体进行了微茎尖组培脱毒研究,探讨了在培养基中添加不同的激素成分及不同浓度对不定芽诱导、继代增殖和生根培养的影响。[结果]欧李茎尖脱毒快繁不定芽诱导的最佳培养基是:MS+6-BA 0.4mg/L+IAA 0.5 mg/L;增殖培养的最适培养基为MS+6-BA 0.3 mg/L+IAA 0.4 mg/L;最佳生根培养基是:2/3MS+IAA 0.5 mg/L。应用指示植物鉴定法进行病毒检测显示,0.3～0.4 mm茎尖培养对苹果褪绿斑病毒(ACLSV)和李矮缩病毒(PDV)的脱毒率分别为71.6%和73.0%。[结论]试验表明,利用欧李嫩梢为外植体进行茎尖组培脱毒快繁是一种可靠实用的方法。[ Objective ] The aim was to study the virvs-free and rapid propagation technique of Prunus humilis （ Bunge. ） [ Method ] Buds or tender stem of Prunus humilis as explant, the micropropagation was studied, the influence of the factors such as different hormone composition, concentration in culture medium upon adventitious bud induction, shoot proliferation and rooting were discussed. [ Result ] The result showed that the optimum medium for adventitious bud regeneration of virus-free and rapid propagation of Prunus humilis was MS ＋ 6-BA 0.4 mg/L ＋ IAA 0.5 mg/L ; the best culture medium for shoot proliferation was MS ＋ 6-BA 0.3 mg/L ＋ IAA 0.4 mg/L ; the proper culture medium of rooting was 2/3 MS ＋ IAA 0.5 mg/L . The virus-free rate of ACLSV and PDV were 71.6% and 73.0% respectively on 0.3 - 0.4 mm shoot tip based on the plant indicator identification, [ Conclusion ] The test showed that the virvs-free and rapid propagation technique of Prunus humilis （Bunge.） were tissue cultured by the method of buds or tender stem of Prunus humilis as explant which is a reliable and adapt method.

关 键 词：欧李 茎尖培养 脱毒 快速繁殖 

分 类 号：S662.3[农业科学—果树学]