新疆沙冬青抗冻蛋白基因保守区序列的克隆及其分析  

Cloning and Sequence Analysis of AFP Gene Conservative Region in Ammopiptanthus Nanus

作　　者：詹彬[1,2] 安沙舟[1] 杨丽娟[2] 李莲瑞[2] 马春晖[2] 

机构地区：[1]新疆农业大学草业与环境科学学院,乌鲁木齐830052 [2]塔里木大学动物科学学院/新疆兵团塔里木畜牧科技重点实验室,新疆阿拉尔843300

出　　处：《新疆农业科学》2009年第6期1307-1310,共4页Xinjiang Agricultural Sciences

基　　金：国家教育部科学技术研究重点项目(206170)

摘　　要：【目的】从Genbank数据库中选择与新疆沙冬青(Ammopiptanthus nanus)亲缘关系最近的同属蒙古沙冬青(Ammopiptanthus mongolicus)抗冻蛋白(AFP)基因AY590122、AY843521、AY843522的mRNA序列,设计扩增新疆沙冬青AFP基因保守区引物。【方法】以新疆沙冬青为研究材料,提取其总RNA,利用RT-PCR技术克隆出新疆沙冬青AFP基因的保守区域。【结果】序列分析表明,新疆沙冬青与蒙古沙冬青的保守区域同源性达98.15%。【结论】得到新疆沙冬青的抗冻蛋白的保守区域。【Objective】A pair of primers were designed according to the three antifreezing proteins（AFP） gene of Ammopiptanthus mongolicus（Genbank accession number is AY590122,AY843521,and AY843522,respectively）.【Method】Total RNA of Ammopiptanthus nanus was extracted using Trizol,which then was used as a template to amplify conservative region of AFP gene by RT-PCR in Ammopiptanthus nanus.【Result】The result showed that the conservative region of AFP gene of Ammopiptanthus nanus had high homology and similarity（98.15%） to the AFP gene of Ammopiptanthus mongolicus.【Conclusion】The above result could also set the base for the complete AFP gene of Ammopiptanthus nanus

关 键 词：新疆沙冬青 抗冻蛋白 克隆 序列分析 

分 类 号：S188[农业科学—农业基础科学]