骨碎补提取工艺的实验研究  

Reserch on Extraction of Drynaria Fortunei(Kunze) J. Sm. with Method of Orthogonal Design

作　　者：全红[1] 郭春秋[1] 曲倩文[1] 王术平[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,黑龙江哈尔滨150040

出　　处：《辽宁中医药大学学报》2009年第12期16-17,共2页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的:优选骨碎补提取的最佳工艺条件。方法:以骨碎补中柚皮苷及骨碎补总黄酮的含量为指标,采用L9(34)正交设计法对骨碎补的提取条件进行研究。结果:提取次数是骨碎补有效成分提取的关键因素,其次是溶媒用量,提取时间和乙醇浓度为最次要因素。结论:最佳提取工艺条件是14倍量55%乙醇回流提取3次,2h/次。Objective：To study the optimum extraction of Drynaria fortunei（Kunze） J. Sm. Methods： Referring to the content of total flavonoids and Naringin, we study the condition of the extraction of Drynaria fortunei（Kunze） J. Sin. with the method of Orthogonal design. Results：The extraction times is the key fator; the dosage of menstruum is the subordination factor; the time cost in extraction and consitence of ethanol is the most subordination factor.Conclusions： The optimum extraction is as follows adding 14 times of 55% ethanol into Drynaria fortunei（Kunze） J. Sm., boiling for 2 hours each time and extracting three times in all

关 键 词：骨碎补 柚皮苷 骨碎补总黄酮 HPLC 正交设计 

分 类 号：G424.31[文化科学—课程与教学论]