白介素-1β对高糖刺激的人肾小管上皮细胞转分化的影响  

THE EFFECT OF INTERLEUKIN-1β ON RENAL TUBULAR EPITHELIAL-MYOFIBROBLAST TRANSDIFFERENTIATION INDUCED BY HIGH GLUCOSE

作　　者：刘青娟[1] 李航[1] 曹延萍[2] 刘淑霞[1] 郝军[1] 吴海江[1] 段惠军[1] 

机构地区：[1]河北医科大学病理教研室,石家庄050017 [2]河北省中医院病理科,石家庄050011

出　　处：《中国组织化学与细胞化学杂志》2009年第6期683-686,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：河北省自然科学基金(C2008001029)

摘　　要：目的探讨炎症细胞因子白介素-1β(interleukin-1βIL-1β)对高糖刺激的人肾小管上皮细胞转分化的影响。-方法体外培养人肾近曲小管上皮细胞株(HKCs),随机分为正常对照组(5.5 mmol/L normal glucose);高糖组(30 mmol/L high glucose);高糖+IL-1β(5ng/ml)组。分别于处理后24h、48h、72h收集细胞,采用免疫细胞化学染色和Western蛋白印迹法检测细胞角蛋白-18(cytokeratin-18 CK-18)、α-平滑肌肌动蛋白(α-smooth muscle actinα-SMA)水平。结果高糖能够诱导肾小管上皮细胞α-SMA蛋白的合成增加,而肾小管上皮细胞的标志物CK-18的表达逐渐减少;IL-1β与高糖同时刺激可使肾小管上皮细胞α-SMA蛋白表达进一步增多,而其自身标志物CK-18的表达则明显下降。结论炎症因子IL-1β能增强高糖对肾小管上皮细胞转分化的作用。Objective To investigate the effect of interleukin-1β on epithelial-myofibroblast transdifferentiation induced by high glucose.MethodsCultured HKCs were divided into three groups： control group（5.5mmol/L normal glucose）,high glucose group（30 mmol/L）,high glucose ＋IL-1β（5ng/ml） group.Cells were collected after 24,48 and 72h of culture.Immunocytochemistry and Western blotting analysis were used to determine the expression of cytokeratin-18（CK-18） and α-smooth muscle actin（α-SMA）.ResultsThe protein level of α-SMA in tubular epithelial cells induced by high glucose was higher than that in control group cells,while the expression of CK-18 was decreased.When stimulated by IL-1βand high glucose simultaneously,HKCs expressed more α-SMA and less CK-18 than that stimulated by high glucose alone.Conclusion IL-1β can enhance the transdifferentiation of renal tubular epithelial cells induced by high glucose.

关 键 词：白介素-1Β 肾小管上皮细胞 表型转化 

分 类 号：R735.2[医药卫生—肿瘤]