牛磺酸对糖尿病大鼠视网膜NO及其合酶影响  

Effect of taurine on expression of nitric oxide and nitric oxide synthase in diabetic rat retina

作　　者：方家华[1] 朱炎华[1] 张祖海[1] 柏晏杰[1] 姚亚春[1] 李静波[1] 

机构地区：[1]长江大学附属荆州市第一人民医院眼科,湖北荆州434000

出　　处：《中国公共卫生》2009年第12期1483-1484,共2页Chinese Journal of Public Health

基　　金：湖北省荆州市科技局基金(20071PE1-37)

摘　　要：目的探讨牛磺酸对糖尿病大鼠视网膜一氧化氮(NO)及其合酶(NOS)表达的影响。方法链脲佐菌素制备大鼠糖尿病模型,24只成年雄性SD大鼠随机分成对照组、糖尿病组、牛磺酸干预组,每组8只。牛磺酸干预组饲料添加1.2%牛磺酸。12周处死,取视网膜标本进行实验。硝酸还原酶测定NO含量,免疫组化法测定神经型一氧化氮合酶(nNOS)及诱导型一氧化氮合酶(iNOS)的表达。结果糖尿病组与牛磺酸干预组NO含量表达为(2.138±0.260)和(1.053±0.159)μmol/L,差异有统计学意义(P<0.01);糖尿病组与牛磺酸干预组iNOS吸光度分别为(0.390±0.022)和(1.101±0.006),后者明显低于前者(P<0.01);牛磺酸干预组nNOS吸光度为(0.429±0.035),明显高于糖尿病组的(0.125±0.024)(P<0.01)。结论牛磺酸通过减少视网膜NO含量和影响iNOS、nNOS表达,改变糖尿病性视网膜病变(DR)。Objective To explore the effect of taurine on the expression of nitric oxide（NO）and nitric oxide synthase（NOS）in diabetic rat retina.Methods A rat model of diabetic retinopathy（DR） was induced by intraperitoneal injection of streptozotocin（STZ）.Twenty-four adult male Sprague-Dewley rats were randomly divided into three groups：normal control guoup,diabetic guoup,taurine-treated diabetic group（dietary supplementation with 1.2% taurine）.The rats were sacrificed at the time of 12 weeks.The content of NO in retina was detected with nitrate reductase method.The distribution and changes of neuronal nitric oxide synthase（nNOS） and inducible nitric oxide synthase（iNOS） were studied with immunohistochemical localization.Results The content of NO in diabetic guoup and taurine-treated diabetic guoup was 2.138±0.260 and 1.053±0.159 μmol/L,respectively.The difference was statistically significant（P〈0.01）.Mean absorbance of iNOS was markedly lower in taurine-treated diabetic guoup than in diabetic guoup（0.101±0.006 vs 0.390±0.022,P〈0.01）.However,mean absorbance of nNOS was significantly higher in taurine-treated diabetic guoup than in diabetic guoup（0.429±0.035 vs.0.125±0.024,P〈0.01）.Conclusion Taurine supplementation ameliorates diabetic retinopathy via downregulating NO and influencing the expression of iNOS and nNOS in rats.

关 键 词：牛磺酸 糖尿病性视网膜病 一氧化氮 一氧化氮合酶 

分 类 号：R587.1[医药卫生—内分泌]