哈蟆油总蛋白提取工艺  

EXTRACTION TECHNOLOGY OF OVIDUCTUS RANAE PROTEINS CONCENTRATION

作　　者：刘诗超[1] 赵雨[2] 张梅[2] 黄萍[3] 金光洙[1] 

机构地区：[1]延边大学药学院,吉林延吉133000 [2]长春中医药大学中医药与生物工程研发中心,吉林长春130117 [3]吉林农业大学中药材学院,吉林长春130118

出　　处：《食品研究与开发》2009年第12期19-21,共3页Food Research and Development

基　　金：国家"十一五"科技支撑项目(2007BAI38B06)

摘　　要：确定哈蟆油主要有效成分哈蟆油蛋白的最佳提取工艺。以蛋白含量为评价指标,采用单因素考察法,筛选出最佳提取条件。哈蟆油蛋白的最佳提取工艺为:浸提液的pH值为6.5,料液比1:200,浸提时间12h,浸提次数3次,浸提液含盐0.5mol/L。验证试验结果表明,最终哈蟆油总蛋白收率为41.27%。To establish the optimal extracting technology of Oviductus Ranae proteins which is the the main active ingredient of Oviductus Ranae. According to the content of protein, a monofactor test was developed to determine the optimum extraction process. The optimum extraction technology was obtained as follows： materialliquid rate （w/v） was 1：200, extracted 12 h three times while the pH value of 6.5 at 0.5 mol/L salinity. The eventually Oviductus Ranae protein yield was 41.27 %.

关 键 词：哈蟆油 总蛋白 提取工艺 

分 类 号：TQ461[化学工程—制药化工]