3种植物Ran基因的克隆及序列分析  

Cloning and Sequence Analysis of Three Plant Ran Genes

作　　者：马立安[1,2] 张忠明[2] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]华中农业大学农业微生物学国家重点实验室,湖北武汉430070

出　　处：《安徽农业科学》2009年第34期17281-17283,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学基金项目(30070370)

摘　　要：[目的]研究洋葱、大蒜和油菜中Ran基因与拟南芥Ran2基因的同源性,以确定3种植物材料能否作为拟南芥的替代材料研究Ran的定位。[方法]采用RT-PCR方法,以拟南芥Ran2的引物分别从洋葱、大蒜和油菜分裂细胞提取的总RNA中克隆出同源基因,进行测序、比对分析。[结果]洋葱、大蒜和油菜的Ran基因开放阅读框分别为666、663和666bp;分别编码221、220和221个氨基酸,分子量约24.3kD;与拟南芥AtRan2氨基酸序列同源性分别为99.1%、100.0%(除末端缺少1个天冬氨酸D外)和96.4%;进化树分析显示洋葱和大蒜Ran基因与拟南芥进化关系更近。[结论]为进一步研究植物Ran基因的生物学功能奠定了基础。[ Objective ] The aim was to study homology between Ran gene in Allium cepa, Allium sativum and Brassica napus and Ran2 gene in Arabidopsis in order to determine whether three kinds of plant material as substitute forArabidopsis. [ Method] By using RT-PCR method, homology gene was cloned from totoal RNA which extracted from splinter cells of AUium cepa, Allium sativum and Brassica napus with Arabidopsis Ran2 primer. Then, carried out sequence and comparative analysis. [ Result] The results showed that the open reading frames of Ran genes in Allium cepa, Allium sativum and Brassica napus were 666,663,666 bp, coding 221,220 and 221 amino acids respectively, with the molecular weight of 24.3 kDa. The sequence analysis showed that the amino acid homology of Ran genes between AUium cepa, Allium sati- vum, Brassica napus and Arabidopsis Ran2 were respectively 99.1% , 100.0% （except an Asp D at C terminal） , 96.4%. The phylogenetic tree indicated that Ran genes from Allium cepa and Allium sativum had closer evolutionary relationship with Arabidopsis Ran2. [ Conclusion ] The research laid a foundation for further study on the biological function of plant Ran gene.

关 键 词：植物Ran 拟南芥Ran2 RT-PCR 序列分析 

分 类 号：S188[农业科学—农业基础科学]