FISH技术和染色体核型分析联合检测体外受精-移植后胎儿与葡萄胎共同妊娠1例  

The combination of FISH technology and chromosome karyotype analysis to detect a case of pregnancy with hydatidiform mole and co-existent live fetus after IVF-ET

作　　者：付娟娟[1] 丛林[1] 袁静[1] 叶四云[1] 方慧琴[1] 

机构地区：[1]安徽医科大学第一附属医院妇产科产前诊断中心,合肥230022

出　　处：《安徽医科大学学报》2009年第6期746-748,共3页Acta Universitatis Medicinalis Anhui

基　　金：卫生部科研基金(编号:WKJ2007-3-001);安徽省2008年科技攻关项目(编号:08010302181);安徽省2008年临床医学应用技术项目(编号:2008A003)

摘　　要：目的探讨胎儿和葡萄胎共同妊娠病例的诊断方法。方法结合超声、临床检查、病理检查、染色体核型分析和荧光原位杂交技术(FISH技术)诊断1例体外受精-移植后胎儿和葡萄胎共同妊娠的病例,并随访患者的预后情况。结果孕妇因阴道流血较多而行钳刮术终止妊娠。刮出物送病理和遗传学分析,病理结果符合葡萄胎的诊断,染色体核型分析和FISH技术的结果相符,均显示胎儿和葡萄胎的染色体均为二倍体,结合病理结果、超声显示和血HCG水平,诊断为完全性葡萄胎与胎儿共存。结论葡萄胎是一种少见的妊娠滋养细胞疾病。可用染色体核型分析和FISH技术检测胎儿是否整倍体,并结合临床特点等决定临床治疗方案。FISH技术所需时间短,便于临床及时诊治。Objective To study the diagnostic methods of pregnancy with hydatidiform mole and co-existent live fetus. Methods Chromosome karyotype analysis and molecular biology method （ FISH technology） were used to diagnose the case of pregnancy with hydatidiform mole and co-existent live fetus after IVF-ET, combining with ultrasound, clinical examination, pathology, and follow-up the results. Results Pregnancy was terminated by curettage of uterus because of the large vaginal bleeding. The tissues of curettage were received pathological detection and ge- netieal analysis. The results of chromosome karyotype analysis and FISH technology were identical which were the chromosome of fetus and hydatidiform mole were diploid. Combining with the outcome of pathology, ultrasound and maternal hCG levels, the complete hydatidiform mole could be diagnosed. Conclusion Hydatidiform mole is one kind of rare gestational trophoblastic diseases. Chromosome karyotype analysis and FISH technology can be used for detecting the chromosome karyotype of fetus whether euploid or not. Clinical treatment is decided by genetical resuits and clinical features. FISH technology can save plenty of time, and it can make a good benefit for clinical diagnosis and treatment in time to combine FISH and prenatal diagnosis.

关 键 词：原位杂交 荧光 葡萄胎 产前诊断 核型分析 胚胎移植 

分 类 号：R714.5[医药卫生—妇产科学] R737.33[医药卫生—临床医学]