Thermal ablation versus conventional regional hyperthermia has greater anti-tumor activity against melanoma in mice by upregulating CD4^+ cells and enhancing IL-2 secretion  

作　　者：Yingying Zhang Wei Zhang Cuanying Geng Tongjun Lin Xiaowen Wang Lingyun Zhao Jintian Tang 

机构地区：[1]Department of Oncology, Xiangya Hospital, Central South University, Changsha 410008, China [2]Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China [3]Clinical Medical Research Center, China-Japan Friendship Hospital, Beijing 100029 China [4]Department of Microbiology ＆ Immunology, Department of Pediatrics, IWK Health Centre, Dalhousie University, 5850 University Avenue, Halifax, NS, Canada B3J 3G9

出　　处：《Progress in Natural Science:Materials International》2009年第12期1699-1704,共6页自然科学进展·国际材料（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.30571779 and 10775085).

摘　　要：To determine whether conventional hyperthermia （42-45℃） or ablation therapy （〉50℃） achieves better synergistic effects on direct cytotoxicity and anti-tumor immunity, we compared the therapeutic effects of two hyperthermia temperatures, 43 and 55 ℃, in terms of cytotoxicity and upregulation of immune functions in a mouse malignant melanoma model. Melanoma-bearing mice were treated by directly applying regional hyperthermia to the tumor nodule with a heating light at a temperature of 43℃ for 30 rain or 55℃ for 10 min. The tumor growth curve and mice survival rate were observed. To investigate the hyperthermia-induced immunological response, peripheral blood CD4^＋ and CD8^＋ T cells and the serum IL-2 level were determined. Our results indicated that application of regional hyperthermia at the ablation temperature （such as 55 ℃） achieved better synergistic anti-tumor effects than did conventional hyperthermia （43℃）. Significant increases in the number of peripheral blood CD4^＋ T cells and the serum IL-2 level likely contributed to the underlying mechanism.为了决定常规过高热( 42 45 °C )或脱离治疗( >50 °C )是否完成更好的 synergistic 效果在上，指导 cytotoxicity 和反肿瘤免疫，我们比较了二过高热温度的治疗学的效果， 43 和 55 °C ，以 cytotoxicity 并且在在一只老鼠的有免疫力的功能的规定上面恶意的黑瘤模型。忍受黑瘤的老鼠被直接为 10 min 为 30 min 或 55 ° C 在 43 ° C 的温度与一盏加热的灯把地区性的过高热用于肿瘤小瘤对待。肿瘤生长曲线和老鼠幸存率被观察。为了调查导致过高热的免疫学的反应，外部血 CD4 ⁺ 和 CD8， ⁺ T 房间和浆液 IL-2 水平被决定。我们的结果在脱离温度显示了地区性的过高热的那应用(例如 55 ° C ) 比完成了更好的 synergistic 反肿瘤效果做了常规过高热(43 ° C ) 。外部血 CD4 ⁺ T 房间和浆液 IL-2 水平的数字的重要增加多半贡献了内在的机制。

关 键 词：Hypertherrnia Thermal ablation MELANOMA Immunity response 

分 类 号：Q78[生物学—分子生物学] TQ463.5[化学工程—制药化工]