应用组织芯片技术研究人类胰腺癌中前列腺干细胞抗原的表达  

Role of prostate stem cell antigen in human pancreatic carcinoma:a tissue microarray-based study

作　　者：杨文彬[1] 蔡锋[2] 程传涛[1] 曹罡[1] 秦兆寅[1] 

机构地区：[1]西安交通大学医学院第二附属医院普外科,陕西西安710004 [2]河南省商丘市第一人民医院普外科,河南商丘476000

出　　处：《南方医科大学学报》2009年第10期2135-2137,共3页Journal of Southern Medical University

摘　　要：目的检测PSCA在胰腺癌中的表达情况,探讨PSCA在胰腺癌发病中所起的作用。方法用组织芯片技术构建包含78例导管腺癌,12例慢性胰腺炎患者,10例正常胰腺组织的100点阵的石蜡组织芯片。用免疫组化SP法检测该芯片中PSCA的表达,分析其与胰腺癌临床病理因素的关系。结果78例胰腺癌患者中,PSCA阳性表达率为79.5%(62/78),与正常组比较PSCA表达与胰腺癌显著相关(χ2=15.81,P<0.005),与慢性胰腺炎比较PSCA亦与胰腺癌显著相关(χ2=11.33,P<0.005);PSCA表达与年龄、性别、组织分化程度及TNM分期无明显相关性。结论PSCA阳性表达与胰腺癌相关,可能与胰腺癌的发生发展有着密切关系,但与胰腺癌的临床病理特型无关。Objective To investigate the expression of prostate stem cell antigen （PSCA） in human pancreatic carcinoma and explore its role in the oneogenesis of pancreatic cancer. Methods A pancreatic carcinoma tissue microarray was constructed, which contained 10 normal adult pancreas tissues, 12 chronic pancreatitis tissues and 78 pancreatic carcinomas. Immunohistochemistry was employed to detect the expression of PSCA, and the relation between PSCA expression and the clinicopathological factors of pancreatic carcinoma was analyzed. Results The positivity rate of PSCA in pancreatic carcinoma was 79.5 % （62/78）, and PSCA staining was more intense in the malignant cells than in the benign cells （X^2=15.81, P〈0.005） and chronic pancreatitis tissues （X^2=-11.33, P〈0.005）. No obvious association was found between PSCA expression and the other variables of pancreatic carcinoma （including gender, age at surgery, tumor grade, and TNM stages）. Conclusion The expression of PSCA can be related to the development of pancreatic cancer, but not to the clinicopathological factors of the tumor.

关 键 词：胰腺癌 PSCA 组织芯片 

分 类 号：R735.9[医药卫生—肿瘤]