苯乙酸钠对小鼠B_(16)黑色素瘤细胞的分化诱导作用  

Differentiation of B_(16) Melanoma Cells Induced by Sodium Phenylacetate

作　　者：叶银英[1] 何道伟[1] 杨天明[2] 丛晓东[3] 

机构地区：[1]南京铁道医学院医学科学研究所,210009 [2]南京铁道医学院第一附属医院 [3]南京中国药科大学

出　　处：《中国肿瘤临床与康复》1998年第4期1-3,共3页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：国家自然科学基金(39570714)资助课题

摘　　要：目的　评价苯乙酸钠对黑色素瘤 B1 6 的抑瘤作用。方法　以 MTT法测定细胞增殖的抑制率。B1 6 胞形态 ,黑色素含量 ,细胞周期变化及体内致瘤能力的测定作为观察指标。结果　用 7.5 m M～ 17.5 m M的苯乙酸钠作用于肿瘤细胞 ,见有不同程度的抑瘤作用 P<0 .0 1。 MTT法测得IC5 0 为 2 .6 7～ 3.6 3m M。在 7.5～ 17.5 m M浓度下 ,对 B1 6 细胞都有较强的分化诱导作用 ,表现为黑色素颗粒生成能力明显增多 ,生长缓慢。细胞动力学研究表明 ,苯乙酸钠可使 B1 6 细胞阻断在 G1 期因而 S期明显减少 ,体内致瘤表明成瘤能力明显降低。结论　苯乙酸钠对 B1 6 细胞有强力分化诱导作用 ,而对体内外黑色素瘤增殖有明显抑制。? Objective:To evaluate the inhibiting effects of sodium phenylacetate on B 16 melanoma cells. Methods:MTT methods was used to determine the inhibiting rate of cell proliferation. The cytomorphological changes of B 16 melanoma cells,melanin content, changes of cell cycle and tumorigenicity in mice were assessed.Results:The sodium phenylacetate at the concentration of 7.5-17.5mM expressed various degrees of inhibition on the growth of B 16 cells (P<0.01).The IC50 was 2.67-3.63mM determined by MTT method.Sodium phenylacetate at concentration of 7.5-17.5mM showed strong induced differentiation on B 16 melanoma cells which expressed productive ability of melanic granule was markedly increased and growth of cells was slowed.The results of cyto dynamics displayed the effect of sodium phenylacetate to block B 16 cells in G1 stage proliferation. The tumorigenicity in vivo was markedly decreased.Conclusion:The results suggest that sodium phenylacetate can strongly induce differentiation of B 16 cells but the growth of B 16 cells in mice or in vitro can be inhibited. 〔

关 键 词：B_(16)黑色素瘤细胞 分化诱导 苯乙酸钠 

分 类 号：R739.502[医药卫生—肿瘤]