罗格列酮对糖尿病兔肾小管上皮细胞表型转化与NADPH氧化酶的影响  

Effect of Rosiglitazone on the phenotypic transformation of renal tubular epithelial cells and NADPH oxidase of diabetic rabbits

作　　者：罗莉[1] 杨立勇[2] 许昌声 

机构地区：[1]福建医科大学附属第一医院干部病房,福建福州350005 [2]福建医科大学附属第一医院内分泌科,福建福州350005 [3]福建省高血压研究所,福建福州350005

出　　处：《中国医药导报》2009年第35期30-31,33,共3页China Medical Herald

摘　　要：目的:研究罗格列酮对NADPH氧化酶表达的影响,探讨其对肾小管上皮细胞表型转化(EMT)的可能作用机制。方法:取28只新西兰大白兔,随机分为对照组(NC组)、糖尿病组(DM组)和罗格列酮干预组(RGT组),DM组和RGT组以四氧嘧啶耳缘静脉注射,RGT组每天给予罗格列酮1.0mg/kg灌胃。第16周末时测定各组血糖、血脂、肌酐清除率(Ccr)、超氧化物歧化酶(SOD);免疫组织化学检测α-平滑肌肌动蛋白(α-SMA),Western印迹检测NADPH氧化酶亚基p22phox的蛋白表达。结果:与NC组比较,DM组Ccr明显升高(P<0.01),SOD明显降低(P<0.05);p22phox和α-SMA蛋白表达升高;与DM组比较,RGT组p22phox和α-SMA蛋白表达降低(P<0.05),而其他指标也有改善。结论:RGT可能通过抑制糖尿病兔肾脏NADPH氧化酶活性,减少肾内氧化应激,阻断α-SMA表达,从而抑制EMT。Objective： To study the effect of Rosiglitazone （RGT） on the expression of NADPH oxidase, and explore the possible mechanism of it on the phenotypic transformation of renal tubular epithelial cells. Methods： 28 New Zealand white rabbits were selected and randomly divided into three groups： NC group, DM group and RGT group, the DM group and RGT group were injected with alloxan via ear vein, and RGT group was given 1.0 mg/kg RGT for intragastric administration once a day. At the end of 16 week, the blood glucose, blood fat, Ccr and SOD of each group were determined, α-SMA was detected by immunohistochemistry, and the protein expression of the subunit p22^phox of NADPH oxidase was detected by Western blot. Results： The Ccr of DM group was significantly higher and the SOD was lower than those of the NC group （P〈0.01, P〈0.05）, and the protein expressions of p22^phox and α-SMA were increased. The protein expressions of p22^phox and α-SMA were lower than those of the DM group （P〈0.05）, and other indexes were also improved. Conclusion： RGT probably inhibits EMT by inhibiting the activity of kidney NADPH oxidase of diabetic rabbits, decreasing the oxidative stress in kidney and further blocking the expression of α-SMA.

关 键 词：罗格列酮 糖尿病兔 肾小管上皮细胞表型转化 NADPH氧化酶 

分 类 号：R-332[医药卫生]