MHCⅠ类链相关蛋白A和基质金属蛋白酶-9在骨肉瘤组织学和血清学中的关系  

Relationship between MICA and MMP-9 in osteosarcoma tissues and patient's sera

作　　者：卢善明[1] 薛玲[2] 邓汪东[3] 曹清华[2] 郑惊雷[4] 车丽洪[2] 

机构地区：[1]汕头大学医学院第一附属医院病理科,汕头515041 [2]中山大学附属第一医院病理科,广州510080 [3]汕头大学医学院第一附属医院泌尿外科,汕头515041 [4]东莞市人民医院肿瘤外科,东莞523018

出　　处：《中国骨与关节外科》2009年第3期206-210,共5页Chinese Journal of Bone and Joint Surgery

基　　金：国家自然科学基金面上项目(30300354)

摘　　要：目的探讨MHCⅠ类链相关蛋白A(MHC classⅠchain-related A,MICA)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在骨肉瘤组织学和血清学中的关系,为研究骨肉瘤组织中MICA蛋白脱落的分子机制提供组织学基础。方法应用免疫组织化学方法检测16例骨肉瘤中MICA和MMP-9蛋白的表达情况,同时采用酶联免疫吸附实验(enzyme-linked immunosorbent assay,ELISA)检测16例骨肉瘤患者血清中MICA和MMP-9的含量。结果16例骨肉瘤组织中MICA和MMP-9蛋白的表达率分别为42.5%(7/16)和56.3%(9/16),两者的表达无相关性(r=0.454,P>0.05);骨肉瘤患者血清中可溶性MICA(soluble MICA,sMICA)浓度高于健康人(P<0.05),与血清中MMP-9的浓度无相关性(r=-0.429,P>0.05),但与组织中MICA蛋白表达呈负相关(r= -0.502,P<0.05)。结论骨肉瘤患者血清中sMICA浓度升高,而血清中MMP-9含量对MICA浓度无明显影响;骨肉瘤患者可能存在NKG2D-MICA介导的免疫监视功能障碍,MICA蛋白的脱落可能是骨肉瘤逃避免疫监视的一个新机制。Objective To investigate the mechanism of the shedding of MHC class I chain - related A （MICA） in osteosarcoma; and the relationship between MICA and matrix metalloproteinase -9 （MMP -9） in osteosarcoma tissues and patient＇s sera was studied. Methods MICA and MMP -9 expression was examined in 16 osteosarcoma tissues by immunohistochemistry. Meanwhile, serum level of soluble MICA （sMICA） and MMP -9 in 16 osteosarcoma patients was evaluated by ELISA. Results MICA and MMP-9 protein were detected in 42. 5% （7/16） and 56.3% （9/16） of osteosarcoma tissues, respectively. MICA expression was not correlated with MMP - 9 expression （ r = 0. 454, P 〉 0. 05）. Serum level of sMICA in osteosarcoma patients was higher than the healthy （ P 〈 0. 05 ） , but it was negatively correlated with MICA expression in osteosarcoma tissues （r = - 0. 502, P 〈 0. 05）. No correlation was found between sMICA and MMP - 9 in patient＇s sera （r = - 0. 429, P 〉 0. 05）. Conclusion Higher serum level of sMICA can be detected in osteosarcoma tissues and can not be affected by the content of MMP - 9 in sera. osteosarcoma patients might have a deficiency of MICA - NKG2D mediated immunosurveillance. The shedding of MICA protein might be a new mechanism of immune evasion for osteosarcoma.

关 键 词：骨肉瘤 MHC Ⅰ类链相关蛋白A MMP-9 

分 类 号：R738.1[医药卫生—肿瘤]