龙眼转醛醇酶基因的克隆与原核表达  

Cloning and Prokaryotic Expression of Transaldolase from Longan

作　　者：游向荣[1] 许鸿川[1] 梁文裕[1] 郑少泉[2] 陈伟[1] 

机构地区：[1]福建农林大学生命科学学院,福建福州350002 [2]福建省农业科学院果树研究所,福建福州350013

出　　处：《北方园艺》2009年第12期7-10,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(30571293);福建省自然科学基金资助项目(2007J0045);教育部博士点基金资助(200803890009)

摘　　要：转醛醇酶作为磷酸戊糖途径非氧化阶段的关键酶,在调节植物PPP对环境胁迫的应答中起重要作用。该研究应用RACE方法克隆了龙眼(Dimocarpus longan Lour.)花芽中转醛醇酶的基因,获得一段长度为1655bp的cDNA,其中包括一个1320bp的开放阅读框,已登陆GenBank,登陆号为FJ472991(GI:217795375)。将转醛醇酶全长cDNA在大肠杆菌中表达,获得一个约55kD带有组氨酸标签的外源蛋白。RT-PCR结果显示,TALmRNA在龙眼成花逆转花芽中有较明显的表达量上调,说明TAL在一定程度上影响了龙眼正常成花。The pentose-phosphate pathway （PPP） plays a pivotal role in carbohydrate metabolism, as a key enzyme of the nonoxidative PPP branch, transaldolase showed advertent response to environmental stress regulation. We have isolated a cDNA encoding transaldolase, an enzyme of the pentose-phosphate pathway, from longan （Dimocarpus longan Lour. ）. RACE method was used and a full length of 1 655 bp cDNA, with a 1 320 bp open reading frame, was obtained （GenBank access number： FJ479618）. Transaldolase cDNA was expressed in E. coli, and a 55 kD heterologous protein with histidine tag was obtained. The RT-PCR result showed TAL mRNA had a remarkable up-regulation, indicate the expression of transaldolase influence longan flowering transition in a certain extent.

关 键 词：龙眼 转醛醇酶 基因 克隆 原核表达 

分 类 号：S667.203.6[农业科学—果树学]