大鼠实验性牙髓炎组织中诱导型一氧化氮合成酶的mRNA表达  

The mRNA expression of the inducible nitric oxide synthase(iNOS) in the experimental rat pulpitis

作　　者：严焱[1] 刘正[1] 

机构地区：[1]上海第二医科大学口腔医学院

出　　处：《牙体牙髓牙周病学杂志》1998年第4期234-235,共2页Chinese Journal of Conservative Dentistry

摘　　要：目的：探讨诱导型一氧化氮合成酶（ｉｎｄｕｃｉｂｌｅｎｉｔｒｉｃｏｘｉｄｅｓｙｎｔｈａｓｅ，ｉＮＯＳ）在牙髓炎症中的ｍＲＮＡ表达。方法：建立大鼠牙髓炎模型，并随机分为４组：正常对照组，牙髓炎２４ｈ、７２ｈ和１２０ｈ组。采用反转录－聚合酶链式反应（ｔｈｅｒｅｖｅｒｓｅｔｒａｎｓｃｒｉｐｔａｓｅ－ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｎ，ＲＴ－ＰＣＲ）方法，测定各组ｉＮＯＳｍＲＮＡ表达量。结果：大鼠牙髓炎早期，局部病灶可见大量中性粒细胞浸润，ｉＮＯＳ表达以各炎症组较对照组升高，其中炎症７２ｈ组明显增高。结论：牙髓炎早期，中性粒细胞可能是ｉＮＯＳ分泌的主要部位，并且ｉＮＯＳ的产生与炎症病程有关。Aim:To detect the mRNA expression of the inducible nitric oxide synthase (iNOS) in the pulpitis. Methods: We established rat pulp inflammation model which was divided into four groups: the control, the pulpitis 24h, 72h and 120h. The mRNA expression of iNOS in all period was investigated by the reverse transcriptase polymerase chain reaction(RT-PCR). Results: In the earlier stage of pulpitis, the local focus was infiltrated by the maximum of the neutrophil leucocytes and the mRNA expression of iNOS in inflamed pulp was higher than that in the control, especially in 72h. Conclusions: The neutrophils may be the capital location secreting iNOS which may has an effect in the development of earlier pulpitis. The production of iNOS may be correlated to the period of disease.

关 键 词：INOS 实验性 牙髓炎 INOSMRNA 病理 

分 类 号：R781.310.2[医药卫生—口腔医学]