大鼠视网膜缺血再灌注损伤中视网膜电图的变化  

Study of ERG changes on retinal ischemic reperfusion injury in rats

作　　者：易有荣[1] 何远[1] 崔冶建[1] 李道全[2] 

机构地区：[1]武汉大学医学院结构研究中心,湖北武汉430071 [2]武汉大学中南医院眼科

出　　处：《临床神经电生理学杂志》2009年第6期328-329,共2页Journal of Clinical Electroneurophysiology

摘　　要：目的：研究视网膜电图（ERG）在视网膜缺血再灌注损伤过程中的变化，探讨其临床应用价值。方法：24只一侧眼视网膜缺血SD大鼠随机分成损伤组、预处理组及丹参组每组8只。损伤组：大鼠视网膜在缺血60min后，分别经再灌注30min、24～72h后测量ERG。预处理组：大鼠视网膜在缺血后60min前24h行5min短暂缺血，再经上述再灌注时程后测量ERG。丹参组：大鼠缺血60min前30min球后注射复方丹参注射液0．05ml，再经上述再灌注时程后测量ERG。结果：损伤组各时程b波下降明显（P〈0．001），预处理组及丹参组再灌注72h后b波恢复（P〉0．05）。结论：视网膜缺血再灌注损伤引起ERG的b波明显下降，ERG的b波是客观反映其损伤及功能恢复的敏感性指标。Objective：To study the changes of ERG on retinal ischemic reperfusion injury in rats. Methods： Twenty-four SD rats were randomly divlded into 3 groups （8 in each）：the injury group, the precondition（PC） group and the radix salviae miliorrhizae（RSM） group. Retinal ischemia was produced for 60 ninutes in anesthetized rats and ERG was measured at 30min,24 h and 72 h later. The rats in PC group underwent retinal ischemia for 5 minutes constituted the PC stimulus and then 60 minutes ischemia 24 h later. The rats in RSM group received a retrohalar injection of salvia miliorrhizae at a dose of 0.05 ml 30 minutes before ischemia. Results： The amplitude of b wave in the injury group declined markedly but completely recovered after 72 h reperfusion in PCgroup and RSM group. Conclusion： Retinal ischemia reperfusion induces amplitude declination of b-wave,and b wave can serve a sensitive assessment of retinal injury and recovery.

关 键 词：视网膜 视网膜电图(ERG) 再灌注损伤 实验 大鼠 

分 类 号：R774.1[医药卫生—眼科] R770.43[医药卫生—临床医学]