肌肽对顺铂所致小鼠急性肾损伤的保护作用  

Protective effect of carnosine against cisplatin-induced acute nephrotoxicity in mice

作　　者：冯震[1] 汪洋[1] 薛晓敏[1] 黄静[1] 马骁骏[1] 吴自荣[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《华东师范大学学报（自然科学版）》2009年第6期32-37,63,共7页Journal of East China Normal University(Natural Science)

基　　金：上海市科委2006年重大项目登山计划(06DZ19016)

摘　　要：研究肌肽(carnosine)对顺铂(cisplatin,CDDP)所致小鼠体内急性肾损伤的保护作用,并探讨可能的机制.雌性昆明小鼠按体重分成4组,分别为正常对照组,肌肽对照组,顺铂化疗模型组和肌肽保护组.正常对照组和肌肽对照组分别连续腹腔注射生理盐水和肌肽10d;顺铂化疗模型组于第5天单次腹腔注射顺铂;肌肽保护组连续腹腔注射肌肽10d,并于第5天单次腹腔注射顺铂;所有动物于第10天采血处死,分别测定各组血清尿素氮(BUN)和肌酐(CRE)含量,分析肾皮质匀浆丙二醛(MDA)、谷胱甘肽(GSH)含量以及超氧化物歧化酶(SOD)的活力变化,苏木精-伊红染色观察肾组织病理学改变.结果表明:顺铂化疗模型组血清CRE,BUN和肾皮质匀浆MDA含量明显上升,GSH和SOD含量显著下降,肾组织病理学变化明显,肌肽保护组可明显改变上述现象.由此得出结论,肌肽可以减轻顺铂引起的急性肾损伤,其作用机制可能与其抗氧化作用和清除自由基活性有密切关系.This paper investigated the protective effect of carnosine on cisplatin （CDDP）-in- duced acute nephrotoxicity in KM mice and the possible plausible mechanism. Forty female KM mice were classified into four groups by weight： control group, carnosine group, CDDP modal group and carnosine prevention group. Normal group and carnosine group were given saline and carnosine intraperitoneally, once daily for 10 days; CDDP modal group was given CDDP intraperitoneally, single dose at the 5th day; carnosine prevention group was given carnosine intraperitoneally, once dialy for 10 days and single dose of cisplatin at the 5th day. All animals were de- capitated at the 10th day, blood serum was collected and analyzed for blood urea nitrogen （BUN） and creatinine （CRE）, the kidney samples were stored for the analysis of malondialdehyde （MDA）, glutathione （GSH）, superoxide dismutase （SOD） and the renal morphology changes by hematoxylin-eosin （HE）staining. As a result, the contents of serum BUN, CRE and kidney tis- sue MDA in the CDDP group increase significantly, while the contents of kidney tissue GSH and SOD decrease strikingly, severe morphology changes is clearly seen. The carnosine prevention group can effectively improve this phenomenon. In conclusion, carnosine can attenuate CDDP-induced acute nephrotoxicity, and the mechanism may be closely involved in antioxidation and scavenging of free radicals.

关 键 词：顺铂 肌肽 急性 肾损伤 氧化应激 

分 类 号：R730[医药卫生—肿瘤]