紫外线致白内障大鼠血清生化指标改变的临床意义  

Clinical Significance of Serum Biochemistry Changing of Cataract Rat Induced by UVB

作　　者：姚雯颖 崔蓓[2] 杜大海[3] 张建荣[3] 李闻捷[3] 

机构地区：[1]武警上海市总队医院检验科,上海201103 [2]第二军医大学附属长海医院眼科,上海200433 [3]第二军医大学附属长海医院实验诊断科,上海200433

出　　处：《现代检验医学杂志》2009年第6期65-67,共3页Journal of Modern Laboratory Medicine

基　　金：国家重大新药创制（专项）资助（2009ZXJ09004-063）.

摘　　要：目的研究紫外线辐射致SD大鼠白内障后，大鼠血清生化指标变化特征。方法将出生后6w体重约180g的24只雄性SD大鼠，每只SD大鼠腹腔注射10g／dI水合氯醛麻醉，扩张瞳孔，置于300nm紫外线灯下，分别照射SD大鼠双眼；裂隙灯观察晶状体损伤；采集大鼠眼球部血，分离、检测血清生化指标。结果①连续辐射7d后可形成典型皮质性白内障；②大鼠血清Ca^2＋，Na^＋，Cl^-，Cu^2＋水平实验组高于对照组；K^＋，Mg^2＋，P，Fe^2＋水平实验组低于对照组；③大鼠血清AKP活性实验组高于对照组；ALT，AST，LDH活性及胆固醇（TC）含量实验组低于对照组。结论紫外线辐射可致SD大鼠白内障，伴发大鼠血清生化离子水平、肝功能相关酶活性及胆固醇水平显著改变。Objective To study the characterization of serum biochemistry indexes of SD rats after UVB radiation. Methods 24 male, 6-week-old SD rats, weighing about 180 g, were treated with intraperitoneal injection, using 10 g/dl Chloral hydrate 0. 6-0. 7 ml （0. 35ml/100g）and placed under 300 nm UVB radiation in the eyes for 15 minutes with papillary dilation once a day, for 7 days; Observed its ultrastructure using transmission electron microscopy and scanning electron micro- scope,removed lenses and collected rat serum for testing. Results （1）Succeeded in constructing cataract animal model after 7 days ultraviolet radiation. （2）Ca^2＋ ,Na^＋ ,Cl^- and Cu^2＋ levels in serum were higher than those in control group,while K^＋, Mg^2＋ ,P and Fe^2＋ levels were lower. （3）AKP activity in serum was higher than that of control group ;ALT,AST and LDH activities and cholesterol content were lower than in control groups. Conclusion Controled UVB radiation causes cortical cataract,accompanied by significant changes.

关 键 词：紫外线 大鼠 白内障 血清生化 

分 类 号：R-332[医药卫生]