DGGE技术在环境微生物多样性研究中的应用  

Application in Research on Microbial Diversity of Environment by DGGE Technique

作　　者：张珍妮[1,2] 吴晓芙[1] 陈永华[1,2] 石卉[1] 

机构地区：[1]中南林业科技大学环境科学与工程研究所,长沙410004 [2]中南林业科技大学生物环境科学与技术研究所,长沙410004

出　　处：《生物技术通报》2009年第12期48-52,共5页Biotechnology Bulletin

基　　金：国家水体污染控制与治理科技重大专项(2008ZX07212-001);国家科技部国际合作项目(2007-DFA91420);湖南省博士后基金(2008RS4027);中南林业科技大学校青年基金重点项目(2008001A);中南林业科技大学生物环境科学与技术研究所开放基金;湖南省环境科学学科建设项目(2006180)

摘　　要：微生物是污水净化的主要作用者之一。采用变性梯度凝胶电泳(denaturing gradient gel electrophresis,DGGE)方法培育和鉴定土壤微生物具有可靠性强、重复性好、方便快捷等优点,已被广泛应用于环境科学和污染防治研究领域。综述了基于PCR-DGGE技术的基本原理、关键环节及其在微生物多样性研究中的应用,同时就其自身存在的不足进行了评价并提出了解决方案。Microorganism plays an important role in wastewater treatment. Accounted for by its high levels of reliability and reproducibility as well as its convenience in operation,the denaturing gradient gel electrophoresis （DGGE）has been widely used for cultivation and identification of microbial communities in the fields of environmental sciences with focus on pollution control. The principle of the DGGE technique and the concerned key factors in its application for analysis of microbial communities were described in this paper. In comparison,the potential application areas of the DGGE technique and its limitations were also discussed.

关 键 词：PCR-DGGE 微生物 多样性 

分 类 号：X172[环境科学与工程—环境科学]