胶束扫集毛细管电泳分离测定绿原酸和咖啡酸  

Simultaneous determination of chlorogenic acid and cafeic acid by sweeping micellar electrokinectic capillary chromatography

作　　者：李利军[1] 郝学超[1] 程昊[1] 吴启涛[1] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006

出　　处：《分析试验室》2009年第12期119-122,共4页Chinese Journal of Analysis Laboratory

摘　　要：采用胶束扫集毛细管电泳分离测定双黄连口服液中的绿原酸和咖啡酸。试验条件为:重力进样时间40 s;以20 mmol/L NaH2PO4,100 mmol/L十二烷基磺酸钠(SDS)为电泳缓冲液(含体积分数15%甲醇,pH 2.20),分离电压-20 kV,检测波长214 nm,讨论了pH、SDS浓度、样品溶剂等对分离效果的影响。在优化条件下,绿原酸和咖啡酸的检出限分别达到1.02和0.168μg/mL,线性范围分别为5.86～51.5μg/mL和1.27～14.5μg/mL。In this work, sweeping micellar electmkinetic chromatography （sweep-MEKC） was used in determination of chlorogenic acid and cafeie acid. The buffer was composed of 20 mmol/L NaH2PO4 and 100 mmol/L SDS containing 15% methanol （V/V） at pH 2.20, separation voltage was - 20 kV, detection wave-length was 214 nm. Under the optimmn conditions, the LODs of chlorogenic acid and cafeic acid were 1.02 μg/mL and 0. 168 μg/mL, the linear ranges were 5.86- 5.15 μg/mL and 1.27- 14.5 μg/mL, respectively.

关 键 词：毛细管电泳 胶束扫集 绿原酸 咖啡酸 

分 类 号：O657.8[理学—分析化学]